荔枝‘妃子笑’AP2同源基因的克隆及表达

Cloning and Expression Profiling of an AP2 Homologous Gene of Litchi‘Feizixiao’

根据笔者课题组荔枝基因组数据库,从‘妃子笑’荔枝中克隆了1个AP2同源基因LcANT,其cDNA全长2087 bp,编码695个氨基酸,含有2个保守的AP2/EREBP结构域。生物信息学分析结果表明,LcANT含有7个内含子,亚细胞定位预测于细胞核,LcANT蛋白为疏水性蛋白,二级结构多为无规则卷曲。通过BLAST比对发现,LcANT与许多植物的ANT转录因子具有高度同源性,与开心果相似度最高,高达82%。利用q−PCR,对LcANT在‘妃子笑’荔枝中的表达模式进行了表征,发现其在春梢、秋梢和花序中表达量较高,暗示其可能参与调控花器官及其他植物营养器官的发育。LcANT基因的获得为后续研究其对荔枝营养器官生长发育的调控作用奠定了基础。

An AP2 homologous gene LcANT was cloned from Litchi‘Feizixiao’(Litchi chinensis Sonn.Feizixiao)based on the litchi whole genome database available from the Environment and Plant Protection Institute.The gene LcANT was 2087 bp in full-length cDNA,encoded 695 amino acids and contained two AP2/EREBP conserved domains.Bioinformatics analysis showed that the LcANT contained 7 introns,and that the subcellular localization was predicted in the nuclear.The LcANT protein was hydrophobic and its secondary structure wass mostly random coils.BLAST sequence alignment analysis showed that the LcANT had a high homology in amino acid sequences with the ANT transcription factors from other plants,and had a maximum identity of up to 82%with the ANT from pistachio(Pistacia vera).The q-PCR was used to characterize the expression pattern of the LcANT in the tissues of Litchi'Feizixiao',and the results showed that the LcANT had higher expression levels in spring shoots,ovary,inflorescence,and flower buds,implying that this gene may regulate the growth and development of floral organs and other plant vegetative organs.The acquisition of the LcANT gene provides a molecular basis for further research focused on its function on the growth and development of vegetative organs of Litchi.