AgNO3逆转的黄芩组培玻璃化苗的活性成分含量及其生物活性研究

Contents of Active Components of Scutellaria baicalensis Plantlet and Its Biological Activities of Hyperhydricityin Tissue Culture Recvered by AgNO3

研究AgNO3对黄芩组培玻璃化苗的逆转作用,并在此基础上对逆转的黄芩组培玻璃化苗的活性成分含量及活性进行分析。结果表明,AgNO3对黄芩的玻璃化苗具有明显的逆转作用,且这种逆转作用受AgNO3浓度的影响。在AgNO3的浓度为4 mg·L^-1时,玻璃化苗的逆转率最高为88%,且逆转的黄芩苗的生长状态最好。在此基础上,对逆转1个月的黄芩玻璃化苗的活性成分含量及生物活性进行研究。逆转的黄芩玻璃化苗体内黄芩苷的含量为68.6 mg·g^-1,明显高于玻璃化苗(38.3 mg·g^-1),但低于正常的组培苗(108.2 mg·g^-1)。此外,提取物的抗氧化、抗菌和抗肿瘤实验结果显示,逆转的黄芩玻璃化组培苗明显好于玻璃化苗,但仍低于正常的组培苗。以上研究结果表明,AgNO3对黄芩组培玻璃化苗具有较好的逆转及恢复作用。

We studied the reversal effect of AgNO3 on the hyperhydric Scutellaria baicalensis plantlets,and the contents of the active components. AgNO3 had a significant recovery effect on biological activities of the restored hyperhydric S. baicalensis plantlets and the recovery effect was affected by the concentration of AgNO3. When the concentration of AgNO3 was 4 mg · L^-1,the recovery rate of hyperhydric plantlets was 88%,and the recovered S. baicalensis plantlets had the best growth state. We studied the contents of active components and biological activities of hyperhydric S. baicalensis plantlets recovered by AgNO3 for one month. The content of baicalin in recovered hyperhydric S. baicalensis plantlets was 68.6 mg·g^-1,significantly higher than that in hyperhydric plantlets( 38.3 mg·g^-1),but lower than that in normal tissue cultured plantlets( 108.2 mg·g^-1).The results of antioxidant,antimicrobial and anti-tumor experiments showed that the recovered hyperhydric S. baicalensis plantlets were significantly better than the hyperhydric plantlets,but still lower than the normal tissue-cultured plantlets. Therefore,AgNO3 had a good recovery effect on the hyperhydric plantlets of S. baicalensis.