重症肌无力患者血清中miR150-5p与IL-10、IL-17水平的相关性研究

Correlation study between serum miR150-5p and IL-10 and IL-17 levels in patients with myasthenia gravis

目的检测miR150-5p在重症肌无力患者血清中的表达水平并分析与炎症因子水平的相关性。方法收集符合本研究要求的重症肌无力患者62例和体检的健康志愿者30例,实时荧光定量聚合酶链反应(qRT-PCR)检测患者血清中miR150-5p的相对表达水平;用酶联免疫吸附试验(ELISA)检测患者血清中炎症因子的水平;采用pearson相关性分析法分析miR-150-5p与炎症因子水平的相关性。结果重症肌无力患者血清中miR-150-5p的相对表达水平明显高于对照组(P<0.001);重症肌无力患者血清中IL-2和IL-17的水平低于对照组(P<0.01),IL-10的水平高于对照组(P<0.01),而IL-19、IL-20和IL-35的水平与对照组比较无明显差异(P>0.05);重症肌无力患者血清中miR-150-5p与IL-10的水平呈正相关(r=0.891,P<0.001),与IL-17的水平呈负相关(r=-0.836,P<0.001);经过药物治疗后重症肌无力患者血清中miR-150-5p和IL-10的水平有所降低(P<0.001),而IL-2和IL-17的水平有所升高(P<0.001)。结论重症肌无力患者中miR-150-5p的相对表达水平增加,且与IL-10的水平呈正相关,IL-17的水平呈负相关。

Objective To detect the expression level of miR150-5 p in serum of patients with myasthenia gravis and analyze its correlation with inflammatory factors.Methods A total of 62 patients with myasthenia gravis who met the requirements of this study were enrolled, and 30 healthy volunteers were examined. Real-time quantitative polymerase chain reaction(qRT-PCR) was used to detect the relative expression level of miR150-5 p in serum. The levels of inflammatory factors in the serum of patients were detected by enzyme-linked immunosorbent assay(ELISA).The correlation between miR-150-5 p and inflammatory factors was detected by pearson correlation analysis. Results The relative expression level of miR-150-5 p in serum of patients with myasthenia gravis was significantly higher than that of the control group(P<0.001).The levels of IL-2 and IL-17 in serum of patients with myasthenia gravis were lower than those of the control group(P<0.01), the level of IL-10 was higher than that of the control group(P<0.01), and there were no significant difference in the levels of IL-19, IL-20 and IL-35 compared with the control group(P>0.05).The relative expression level of miR-150-5 p in serum of patients with myasthenia gravis was positively correlated with the expression level of IL-10(r=0.891, P<0.001), and negatively correlated with the expression level of IL-17(r=-0.836, P<0.001). After drug treatment, the expression levels of miR-150-5 p and IL-10 in serum of patients with myasthenia gravis decreased(P<0.001), and the levels of IL-2 and IL-17 increased(P<0.001).Conclusion The relative expression level of miR-150-5 p was increased in patients with myasthenia gravis and was positively correlated with the expression level of IL-10 and negatively correlated with the expression level of IL-17.