止血镇痛胶囊对缩宫素致大鼠离体子宫平滑肌收缩的影响

Effect of Zhixue Zhentong Capsule on contraction of isolated uterine smooth muscle induced by oxytocin in rats

目的探讨止血镇痛胶囊对缩宫素致大鼠离体子宫平滑肌收缩的影响。方法选取SPF级雌性SD大鼠60只,将大鼠随机分为空白对照组、模型对照组、黄体酮对照组、独一味胶囊不同剂量对照组(0.2494、0.4975、0.9901、1.9608、3.8461 mg胶囊内容物/mL)、止血镇痛胶囊不同剂量组(0.2494、0.4975、0.9901、1.9608、3.8461 mg胶囊内容物/mL),每组各10~15只。大鼠腹腔注射苯甲酸雌二醇致敏,48小时后处死取子宫平滑肌,并加入缩宫素致其收缩,最后依次加入5个终浓度(0.2494、0.4975、0.9901、1.9608、3.8461 mg胶囊内容物/mL)的止血镇痛胶囊溶液。记录给药前后子宫平滑肌的收缩变化,分别对平均张力值、收缩频率、活动度进行分析。结果止血镇痛胶囊对空白对照组大鼠离体子宫平滑肌收缩的平均张力、频率和活动度均无明显影响(P>0.05)。模型对照组平均张力值明显高于空白对照组(P<0.05),但两组频率和活动度比较差异无统计学意义(P>0.05)。止血镇痛胶囊、独一味胶囊溶液3.8461 mg平均张力值均明显低于模型对照组(P<0.05),但此两种胶囊溶液5个浓度组的频率和活动度与模型对照组比较差异均无统计学意义(P>0.05)。止血镇痛胶囊(3.8461 mg胶囊内容物/mL)能明显抑制缩宫素致大鼠子宫平滑肌收缩,表现为平均张力降低(P<0.05),但对收缩频率和活动度均无明显影响(P>0.05)。止血血镇痛胶囊各浓度组和独一味胶囊各浓度组比较差异均无统计学意义(P>0.05)。结论止血镇痛胶囊能直接抑制大鼠离体子宫平滑肌的收缩,表现为平均张力降低,但对收缩频率和活动度无明显影响,可能是其临床治疗痛经的机制之一。

Objective To investigate the effect of Zhixue Zhentong Capsule on the contraction of isolated uterine smooth muscle in rats.Methods 60 SPF female SD rats were randomly divided into blank control group,model control group,progesterone control group,different dose control groups of Duyiwei Capsule(0.2494,0.4975,0.9901,1.9608,3.8461 mg capsule contents/mL),different dose groups of Zhixue Zhentong Capsule(0.2494,0.4975,0.9901,1.9608,3.8461 mg capsule contents/mL),10-15 in each group.Rats were intraperitoneally injected with estradiol benzoate for allergic sensitization.After 48 h,the rats were killed and the smooth muscle of the uterus was removed,and then oxytocin was added to induce constriction in the smooth muscle of the uterus.Finally,five different final concentrations(0.2494,0.4975,0.9901,1.9608,3.8461 mg capsule contents/mL)of Zhixue Zhentong Capsule were added successively.The contractile changes of uterine smooth muscle before and after administration were recorded,and the mean tension,contractile frequency and activity were analyzed.Results No significant effect of Zhixue Zhentong Capsule on the contraction of uterine smooth muscle was observed in terms of the average tension,frequency and activity(P>0.05).Zhixue Zhentong Capsule(3.8461 mg capsule content/mL)can significantly inhibit oxytocin-induced contraction of uterine smooth muscle in rats,showing a decrease in average tension(P<0.05),but no significant effect was noticed on contraction frequency and activity(P>0.05).Conclusion Zhixue Zhentong Capsule can directly inhibit the contraction of isolated uterine smooth muscle in rats,which could be one of the mechanisms for clinical treatment of dysmenorrhea.