樱桃核的主要成分及水溶性多糖的体外抗氧化测定

Detection of main components and antioxidant activity of water-soluble polysaccharides in cherry nuts

樱桃加工过程产生的大量加工副产物--樱桃核会被丢弃。对樱桃核主要成分的含量进行了测定。采用水溶醇沉方法从樱桃核中提取水溶性多糖,并进行了体外抗氧化功效评价。实验测得樱桃核中主要成分含量(干重)依次为:蛋白质含量18.80±0.30 g/100 g,脂质含量8.97±0.58 g/100 g,可溶性总糖含量4.96±0.14 g/100 g,灰分含量1.49±0.08 g/100 g,维生素C(Vc)含量27.55±0.99 mg/100 g。樱桃核水溶性多糖对1,1-二苯基-2-三硝基苯肼(DPPH)、2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、羟基自由基的体外清除能力非常稳定,IC50分别为0.31、0.32和0.34 mg/mL。樱桃核具有一定的营养价值和抗氧化功效,为樱桃核的"变废为宝"开发利用提供理论参考。

Cherry nuts,as by-products of processing cherry,were always discarded.Main components of cherry nuts were analyzed.The water-soluble polysaccharides from cherry nuts were extracted by water soluble alcohol precipitation method,and then the antioxidant activity was evaluated in vitro.The results showed that the dry weight contents of the protein,lipid,total soluble saccharides,ash and Vc were 18.80±0.30 g/100 g,8.97±0.58 g/100 g,4.96±0.14 g/100 g,1.49±0.08 g/100 g and 27.55±0.99 mg/100 g,respectively.The water-soluble polysaccharides extraction had stable scavenging ability against 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·),2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical(ABTS·) and hydroxyl radical(·OH).When the scavenging rates against DPPH·,ABTS·and·OH were 50%,the concentrations of water-soluble polysaccharides were 0.31,0.32 and 0.34 mg/mL,respectively.The cherry nuts had certain nutritional value and antioxidant function.This research provided theoretical basis for the further development and utilization of cherry nuts,processing waste into useful material.