摘要
为研究大叶糖胶树(Alstonia macrophylla)化学成分及生物活性。采用正相硅胶、葡聚糖凝胶、制备液相等色谱技术,对大叶糖胶树中的化学成分进行了分离纯化,共分离得到6个化合物,通过NMR、MS等波谱方法将其结构鉴定为vallesamine(1)、picrinine(2)、khasuanine A(3)、scholaricine(4)、echitamine(5)、strictosamide(6)。化合物3和6首次从该属植物中分离得到,化合物1、2、4、5为首次从该植物中分离得到。所有化合物测试了对HCT-116人结肠癌细胞和DLD-1人结直肠腺癌上皮细胞的细胞毒性,结果显示化合物2和4具有一定的细胞毒性,化合物4对DLD-1人结直肠腺癌上皮细胞的细胞毒性较为明显(IC50 =16.7μg/mL),其余化合物均未显示出生物活性。
In order to investigate the chemical constituents and biological activities of Alstonia macrophylla,six monoterpenoid indole alkaloids,namely vallesamine(1),picrinine(2),khasuanine A(3),scholaricine(4),echitamine(5),strictosamide(6) were isolated from leaves and twigs of Alstonia macrophylla by repeated column chromatography over silica gel,Sephadex G and Prep-HPLC.Their structures were elucidated based on NMR and MS spectroscopic data.Compounds 3 and 6 were firstly obtained from this genus,and compounds 1,2,4 and 5 were isolated from this plant for the first time.All compounds were evaluated the cytotoxicity of HCT-116 and DLD-1.Among them,compounds 2 and 4 had certain cytotoxicity,and compound 4 showed DLD-1 inhibitory activity with an IC50 value of 16.7 μg/mL.
作者
张金苹
闫慧娇
王亚兰
王志伟
王晓静
ZHANG Jin-ping;YAN Hui-jiao;WANG Ya-lan;WANG Zhi-wei;WANG Xiao-jing(School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250200,China;Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 2500140,China;Institute of Materia Medica,Shandong Academy of Medical Sciences;Key Laboratory for Biotech-Drugs Ministry of Health,Key Laboratory forRare-Uncommon Diseases of Shandong Province,Jinan 2500620,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第4期622-626,共5页
Natural Product Research and Development
基金
国家自然科学基金(31700290)。
关键词
大叶糖胶树
吲哚生物碱
生物活性
化学成分
Alstonia macrophylla
indole alkaloids
bioactivity
chemical constituents