车前子对腹泻大鼠炎性因子和结肠组织 AQP8蛋白表达的影响

Effects of Plantago asiatica L.on inflammatory cytokines and expressions of AQP8 gene and protein in colon tissue of diarrhea rats

为了观察车前子对番泻叶诱导的腹泻大鼠血清中炎性因子和结肠组织中水通道蛋白8(AQP8)蛋白表达的调控作用,实验将60只SD雄性大鼠随机分为正常组、模型组、氢氯噻嗪组(9 mg/kg)和车前子高、中、低剂量组(3.8、1.9、0.95 g生药/kg),每组10只。除正常组外,其余各组均于每日上午灌胃番泻叶药液(20 mL/kg),正常组灌胃等量的蒸馏水;每日下午各治疗组灌服相应药物,正常组和模型组灌服等量的蒸馏水,连续14天。实验期间每天观察大鼠排便情况,实验结束后,收集血清、结肠组织标本检测血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和C反应蛋白(CRP)的含量;苏木精-伊红(HE)染色法观察病理形态学变化;免疫组化法和Western blot法检测结肠组织中AQP8蛋白表达情况。结果显示车前子各剂量组均能改变大鼠粪便性状,大便基本成形;降低血清中TNF-α、IL-6和CRP的含量;减轻结肠黏膜的病理损害;上调AQP8蛋白的表达。上述研究结果表明,车前子能防治番泻叶所致的腹泻,其作用机制可能与抑制炎症反应、修复结肠黏膜病理损伤,上调AQP8蛋白表达,促进水液代谢有关。

To explore the regulatory effect of Plantago asiatica L.on expressions of AQP8 in colon tissue and serum inflammatory factors in Senna-triggered diarrhea rats.60 Sprague-Dawley male rats were randomly divided into normal group,model group,hydrochlorothiazide group( 9 mg/kg), and high,middle,and low Plantago asiatica L.groups( 3.8,1.9,0.95 g crude drugs/kg,respectively),with 10 rats distributed in each group.Intragastric administration of Senna( 20 mL/kg) was implemented in the morning for 5 groups except of normal group,which was implemented by same dose of distilled water.In the afternoon,each treatment group was given corresponding drugs,while normal group and model group were given same dose of distilled water for 14 consecutive days.Defecation of rats was detected during administration.At the end of experiment,samples collected from serum and colon tissue of rats were provided for content detection of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and C-reactive protein(CRP);HE staining was applied to observe the pathomorphological changes,while immunohistochemical method and Western blot were used to detect protein expressions of AQP8 in colon tissue.The results showed that every group of Plantago asiatica L.changed the fecal characteristics of rats,and the stools were basically formed;Contents of serum TNF-α,IL-6 and CRP decreased,and pathological damage of colonic mucosa was reduced,and expression of AQP8 protein was elevated.Conclusion could be achieved that Plantago asiatica L. can prevent diarrhea induced by Senna,and its mechanism may be related to inhibition of inflammatory reaction,repair of pathological damage of colonic mucosa,up-regulation of AQP8 expression and promotion of water and fluid metabolism.