狭叶小金梅草组织培养与快速繁殖

In vitro Culture and Rapid Propagation of Hypoxis angustifolia

以狭叶小金梅草(Hypoxis angustifolia)当年花苞为材料进行离体快速繁殖体系研究。结果表明,花苞的愈伤诱导适宜培养基为MS+6-BA 0.8 mg·L^-1+NAA 0.05 mg·L^-1+TDZ 0.1 mg·L^-1;诱导芽培养基为MS+6-BA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC(活性碳)0.2 g·L^-1;芽继代增殖培养基为MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+花宝1号1 g·L^-1+AC 0.2 g·L^-1,30 d为一个继代周期,增殖系数达10~20倍;壮苗生根培养基为1/2MS+IBA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC 0.5 g·L^-1。将生根苗移栽至泥土:蛭石按3:1配制旳基质中,盖膜保湿,成活率达80%。

The buds of Hypoxis angustifolia were used as material for tissue culturing.The results showed that the optimum culture medium for bud callus induction was MS+6-BA 0.8 mg·L^-1+NAA 0.05 mg·L^-1+TDZ 0.1 mg·L^-1;the culture medium for bud induction was MS+6-BA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC 0.2 g·L^-1;the culture medium for propagation was MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+Huabao-11 g·L^-1+AC 0.2 g·L^-1.And the subculture circle was 30 d,the multiplication rate was 10-20.The culture medium for root was 1/2 MS+IBA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC 0.5 g·L^-1.The suitable transplanting medium was surface soil and vermiculite(3:1),the survival rate reached 80%.