大鼠建立气管异位移植慢性闭塞性细支气管炎模型后的病理变化及白细胞介素-17的表达

Pathological changes and expression of interleukin-17 in rat model of chronic obliterative bronchiolitis after heterotopic tracheal transplantation

目的探讨大鼠建立气管异位移植慢性闭塞性细支气管炎模型后的病理变化及不同阶段白细胞介素-17(IL-17)的表达情况。方法选取24只清洁级SD大鼠,其中SPF级近交系Brown Norway(BN)大鼠6只,Lewis大鼠18只,随机选取12只Lewis大鼠纳入对照组,将剩余的6只Lewis大鼠与6只BN大鼠纳入观察组。观察组BN大鼠提供气管,Lewis大鼠接受气管;对照组的12只Lewis大鼠既是供体也是受体。比较两组大鼠的软骨损伤率、淋巴细胞计数、大鼠闭塞性细支气管炎的发生率以及IL-17水平。结果移植术后,观察组大鼠闭塞性细支气管炎的发生率为100.00%,高于对照组的0.00%,差异有统计学意义(P<0.05)。两组大鼠移植后第7天的软骨损伤率比较,差异无统计学意义(P>0.05);观察组大鼠移植后第14、28天的软骨损伤率高于对照组,差异有统计学意义(P<0.05);观察组大鼠移植第7、14、28天后的淋巴细胞计数多于对照组,差异有统计学意义(P<0.05)。两组大鼠移植后第7、14天的IL-17水平比较,差异无统计学意义(P>0.05);观察组大鼠移植后第28天的IL-17水平高于对照组,差异有统计学意义(P<0.05)。结论通过建立大鼠异位气管慢性排斥反应模型,提示异位气管移植后,大鼠的IL-17水平升高,有关IL-17导致的闭塞性炎性反应机制还应继续试验与研究。

Objective To investigate the pathological changes and the expression of IL-17(IL-17) in different stages of chronic bronchiolitis obliterans model after heterotopic tracheal transplantation in rats. Methods Twenty-four clean-grade SD rats were selected, of which 6 were brown Norway(BN) and 18 were Lewis. Twelve Lewis rats were randomly selected into the control group, and the remaining six Lewis rats and six BN rats were included into the observation group. The BN rats in observation group provided trachea, Lewis rats received trachea;and the 12 Lewis rats in control group were both donor and receptor. The cartilage injury rate, lymphocyte count, incidence of bronchiolitis obliterans and the level of IL-17 were compared between the two groups. Results After transplantation, the incidence of bronchiolitis obliterans in the observation group was 100.00%, higher than that in the control group(0.00%), with statistically significant difference(P<0.05). There was no statistically significant difference in the cartilage damage rate between the two groups on the 7 thday after transplantation(P>0.05). On the 14 thand 28 thdays after transplantation, the cartilage damage rates in the observation group were higher than those in the control group, with statistically significant differences(P<0.05). After 7, 14 and 28 days of transplantation, the lymphocyte counts in the observation group were more than those in the control group, with statistically significant differences(P<0.05). There were no statistically significant differences in the levels of IL-17 between the two groups on the 7 thand 14 thdays after transplantation(P>0.05). The level of IL-17 in the observation group was higher than that in the control group on the 28 thday after transplantation,with statistically significant difference(P<0.05). Conclusion Through the establishment of the model of chronic rejection of ectopic trachea in rats, it is suggested that the level of IL-17 in rats increased after the transplantation of ectopic trachea, and the mechanism of occlusive inflammatory response induced by IL-17 should be further studied.