丝氨酸蛋白酶1在胃癌组织中的表达及其影响因素分析

Expression of serine protease 1 in gastric cancer and its influencing fac-tors

目的探讨胃癌患者癌组织中丝氨酸蛋白酶1(HTRA1)的水平变化及其影响因素。方法选取2016年7月~2019年7月于我院临床确诊的220例胃癌患者作为研究对象,采用免疫组织化学方法检测患者胃癌组织及远癌组织中的HTRA1表达情况,并分析HTRA1表达与胃癌患者临床特征的关系。结果 HTRA1蛋白在胃癌组织中的阳性表达率(13.18%)低于远癌组织(86.82%),差异有统计学意义(P<0.05);HTRA1蛋白表达阳性与阴性患者的肿瘤直径、浸润深度、吸烟史、酗酒史及饮食习惯比较,差异有统计学意义(P<0.05);HTRA1蛋白表达阳性与阴性患者的年龄、性别、组织分化程度、淋巴结转移及TNM分期比较,差异无统计学意义(P>0.05);Logistic回归分析结果显示,肿瘤直径(β=0.532,OR=1.873,95%CI 0.372~2.711)、浸润深度(β=0.837,OR=1.103,95%CI 0.822~2.014)、吸烟史(β=0.486,OR=1.569,95%CI 1.023~2.832)、酗酒史(β=0.590,OR=1.690,95%CI 0.934~3.721)、饮食习惯(β=0.829,OR=1.552,95%CI 1.032~2.831)均是影响胃癌组织中HTRA1蛋白表达的独立危险因素(P<0.05)。结论胃癌中HTRA1蛋白表达与肿瘤直径、浸润深度、吸烟史、酗酒史及饮食习惯有关,肿瘤直径、浸润深度、吸烟史、酗酒史、饮食习惯均是影响胃癌的危险因素。

Objective To investigate the changes of serine protease 1(HTRA1) in gastric cancer patients and the influencing factors. Methods A total of 220 patients with gastric cancer clinically diagnosed in our hospital from July 2016 to July 2019 were selected as study objects. The expression of HTRA1 in gastric cancer and distant cancer was detected by immunohistochemistry, and the relationship between HTRA1 expression and clinical characteristics of gastric cancer was analyzed. Results The positive expression rate of HTRA1 protein in gastric cancer tissues(13.18%) was lower than that in distant cancer tissues(86.82%), the difference was statistically significant(P>0.05). The differences in tumor diameter, invasion depth, smoking history, drinking history and mental state between positive and negative HTRA1 protein expression patients were statistically significant(P<0.05), while the difference in age, gender, tissue differentiation degree, lymph node metastasis and TNM stage between positive and negative HTRA1 protein expression patients were not statistically significant(P>0.05). Logistic regression analysis showed that tumor diameter(β=0.532,OR=1.873, 95%CI 0.372-2.711), invasion depth(β=0.837, OR=1.103, 95%CI 0.822-2.014), smoking history(β=0.486,OR=1.569, 95%CI 1.023-2.832), drinking history(β=0.590, OR=1.690, 95%CI 0.934-3.721) and mental state(β=0.829, OR=1.552, 95%CI 1.032-2.831) were independent risk factors affecting gastric cancer(P<0.05). Conclusion HRA1 protein expression in gastric cancer is related to tumor diameter, invasion depth, smoking history, drinking history and mental state. Tumor diameter, invasion depth, smoking history, drinking history and mental state are all risk factors affecting gastric cancer.