miR-26b-3p靶向调控TRA2B表达抑制神经胶质瘤细胞的增殖和转移

MiR-26b-3p targeted regulationof TRA2B expression inhibits proliferation and metastasis of glioma cells

目的探讨miR-26b-3p在神经胶质瘤细胞中的表达,以及其对神经胶质瘤细胞增殖和转移的影响。方法采用荧光实时定量PCR(qRT-PCR)检测神经胶质瘤组织与癌旁组织中miR-26b-3p与TRA2B的表达水平;通过向神经胶质瘤细胞细胞系中转染miR-26b-3p mimic和inhibitor干扰内源miR-26b-3p的表达,同时检测其靶基因TRA2B蛋白的表达变化;采用CCK-8法检测各组神经胶质瘤细胞增殖能力的变化,以及划痕实验对癌细胞迁移能力进行评估。结果神经胶质瘤患者组织与癌旁组织比较,miR-26b-3p的表达水平显著上调,TRA2B的表达水平显著下降,差异有统计学意义(P<0.05);Pearson相关性分析显示,在32例神经胶质瘤患者神经胶质瘤组织中,TRA2B的表达水平与miR-26b-3p表达呈显著负相关(r=-0.510;P<0.05);SHG-44细胞体外转染实验结果发现,降低细胞内源miR-26b-3p的表达时,TRA2B的表达水平显著升高,细胞的增殖活性以及转移能力会被抑制;而上调细胞内源miR-26b-3p的表达时,TRA2B的表达水平显著下降,细胞的增殖活性与转移能力会显著提高,相比于对照组,差异有统计学意义(P<0.05)。结论 miR-26b-3p在神经胶质瘤组织中高表达,其可能靶向调控TRA2B的表达抑制神经胶质瘤细胞的增殖活性与转移能力,在神经胶质瘤的发生发展过程中起着重要的生理功能。

Objective To investigate the expression of miR-26 b-3 p in glioma cells and its effect on the proliferation and metastasis of glioma cells. Methods Quantitative real-time polymerase chain reaction(q RT-PCR)was used to detect the expression of miR-26 b-3 p and TRA2 B in glioma and precancerous tissues.By transfection of miR-26 b-3 p mimic into glioma cell lines or using inhibitors inhibit the expression of endogenous miR-26 b-3 p,the expression of TRA2 B protein was determined. The CCK-8 method was used to detect the proliferation of glioma cells in each group. The wound healing assay was used to measure the cancer cell migration. Results The expression level of miR-26 b-3 p was significantly up-regulated,and that of TRA2 B was significantly decreased(P<0.05) in glioma tissues compared with adjacent tissues. Pearson correlation score showed that the expression level of TRA2 B was negatively correlated with the expression of miR-26 b-3 p in 32 cases of glioma(r=-0.510;P<0.05). The results of SHG-44 cell transfection experiments in vitro found that when the expression of miR-26 b-3 p was reduced,the expression level of TRA2 B was significantly increased,and the proliferation and metastasis of cells were inhibited. Incontrast,When up-regulating the expression of endogenous miR-26 b-3 p in cells,the expression level of TRA2 B decreased significantly, and the cell proliferation activity and metastasis ability increased significantly(P<0.05).Conclusion miR-26 b-3 p is highly expressed in gliomas,which may target the regulation of TRA2 B expression and inhibit the proliferation and metastasis of glioma cells. MiR-26 b-3 p plays an important physiological role in the development of gliomas.