基于微生物快速鉴定系统对金黄色葡萄球菌耐药性鉴定新评估方法的建立

Establishment of a New Evaluation Method for Identification of Drug Resistance of Staphylococcus aureus Based on Rapid Identification System of Microorganisms

目的:探讨微生物快速鉴定系统(MALDI-Biotyper System)用于快速鉴定铜绿假单胞菌(Pseudomonas aeruginosa,P.a),临床分离金黄色葡萄球菌(Staphylococcus aureus,S.a)耐药性情况的可行性。方法:通过采用微生物快速鉴定系统及肉汤稀释法进行鉴定质控、临床分离金黄色葡萄球菌的耐药性情况,并将微生物快速鉴定系统检测结果与肉汤稀释法最低抑菌浓度(MIC)值进行比较分析,最后通过同步鉴定铜绿假单胞菌耐药性情况确定微生物快速鉴定系统的准确性及适用性。结果:微生物快速鉴定系统评分鉴定结果显示敏感质控菌株金黄色葡萄球菌评分值大于2.000,耐药质控菌株耐甲氧西林金黄色葡萄球菌(methicillin-resistant S.a,MRSA)评分值在1.700~2.000。临床分离金葡菌评分鉴定结果均在1.700~2.000,显示耐药,与肉汤稀释法MIC值结果一致。同时,无关质控敏感菌株铜绿假单胞菌的系统评分鉴定值大于2.000,显示敏感,其本身为敏感菌株,结果一致。结论:微生物快速鉴定系统评分方法可以用于金黄色葡萄球菌及铜绿假单胞菌耐药性情况的快速鉴定。

Objective:To explore the feasibility of the rapid identification system(MALDI-Biotyper System)of microorganisms for rapid identification of Pseudomonas aeruginosa and clinical isolation of Staphylococcus aureus.Method:Identification quality control and clinical isolation were conducted for drug resistance of S.aureus by microbial rapid identification system and broth dilution method.The scores of microbial rapid identification system were compared with the MIC value of broth dilution method.The drug resistance of P.aeruginosa was simultaneously identified to determine the accuracy and applicability of the rapid identification system of microorganisms.Result:The scores of the microbial rapid identification system showed that the score of sensitive quality control strain S.aureus was higher than 2.000,and the that of resistant strain of methicillinresistant S.aureus(methicillin-resistant S.aureus,MRSA)was between 1.700 and 2.000.The score of clinically isolated S.aureus was between 1.700 and 2.000,which suggested the drug resistance and was consistent with the MIC value of the broth dilution method.At the same time,the systemic identification value of the P.aeruginosa,which is independent of the quality control sensitive strain,was greater than 2.000,showing sensitivity and it was a sensitive strain itself,which was consistent with the results.Conclusion:The microbial rapid identification system scoring method can be used for the rapid identification of the drug resistance of S.aureus and P.aeruginosa.