生物滴滤塔去除硫醇和BTEX的性能及特性研究

Performance and characteristics of biotrickling filter for simultaneous removal of mercaptans and BTEX

针对石油化工行业排放的恶臭硫醇和苯、甲苯、乙苯及二甲苯(benzene,toluene,ethyl benzene and xylenen,BTEX)污染物,采用生物法对其进行去除。在生物滴滤塔(biotrickling filter,BTF)中接种二甲苯降解菌Pandoraea sp.WL1和硫醇降解菌Pseudomonas sp.WL2,对硫醇和BTEX的去除性能、动力学特性及微生物群落特性进行详细研究。运行结果表明,优选菌的接种能大大缩短BTF的启动时间。宏观动力学分析表明,该体系混合VOCs的最大去除能力为210.7g·m^-3·h^-1。16S rRNA测序表明,在经过长时间混合底物的通入后,Hydrogenophaga(17.2%)和Pseudomonas(8.1%)取代Pandoraea成为BTF内的主导菌属。Thiobacillus(4.5%)和Pseudomonas(8.1%)菌属在恶臭硫醇的生物降解中起关键作用。

Biotreatment was used to remove malodorous mercaptans,benzene,toluene,ethylbenzene and xylene(BTEX)discharged from petrochemical industry.The removal performance of mercaptans and BTEX,macro-kinetics and microbial community characteristics were studied in the biotrickling filter(BTF)inoculated with mercaptans degrading bacteria Pandoraea sp.WL1 and BTEX degrading bacteria Pseudomonas sp.WL2.The removal performance results show that the inoculation of the specific bacteria can greatly shorten the start-up time of the BTF.Macro-kinetics analysis indicates that the maximum elimination capacity for the multiple VOC mixture is 210.7 g·m^-3·h^-1.By constructing the 16S rRNA gene clone library,it shows that the relative abundance of BTEX-degrading Pandoraea decreased significantly from 60.0%to 0.5%after a long-term continuous operation,while Hydrogenophaga(17.2%)and Pseudomonas(8.1%)became the dominant genera.Thiobacillus(4.5%)and Pseudomonas(8.1%)play key roles in the biodegradation of the odorous mercaptans.