摘要
目的:筛选大鼠血浆辐射敏感代谢物并探索其代谢通路,为辐射损伤生物标志物研究提供科学依据。方法:用60Coγ射线对20只大鼠进行全身照射,照射剂量分别为0、1、3、5 Gy,剂量率为1 Gy/min,采用基于液相色谱质谱串联平台的非靶向代谢组学方法,检测大鼠受照7 d后血浆中代谢物的变化。结果:大鼠受照后,筛选出血浆中11个辐射敏感代谢物,主要涉及牛磺酸和亚牛磺酸代谢、烟酸和烟酰胺代谢、初级胆汁酸生物合成等代谢通路。十八二烯酰基肉碱、十八一烯酰基肉碱、十六酰基肉碱、牛磺酸、L-犬尿氨酸及胞嘧啶随受照剂量的上升而显著下降(P<0.05),具有明显的剂量-效应关系。结论:大鼠受到γ射线照射后,血浆中代谢物水平发生显著变化,有6个代谢物具备成为辐射生物剂量计的基础条件。
OBJECTIVE:To screen for radiation-sensitive metabolites in rat plasma and to explore themetabolic pathways that provide scientific basis for identification of radiation-sensitive biomarkers. METHODS:Rats irradiated whole-body with60 Co gamma rays of 0,1,3 and 5 Gy,respectively. Changes of metabolitesin rat plasma were detected by non-targeted metabolomics method based on the liquid chromatography massspectrometry platform. RESULTS: Eleven plasma metabolites were identified as the potential radiationbiomarkers. Taurine and hypotaurine metabolism,nicotinic acid and nicotinamide metabolism,and primary bileacid biosynthesis were enriched after irradiation. Linoelaidylcarnitine,11 Z-octadecenylcarnitine,L-palmitoylcarnitine,taurine, niacinamide, L-kynurenine and cytosine were decreased with the increased dose and had gooddose-response relationships. CONCLUSION: The metabolites levels in rat plasma were significantly changedafter exposure to gamma-rays. Six metabolites had the basic conditions for radiation biological dosimeters.
作者
赵骅
陆雪
蔡恬静
李爽
田梅
刘青杰
ZHAO Hua;LU Xue;CAI Tianjing;LI Shuang;TIAN Mei;LIU Qingjie(National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,China CDC Key Laboratory of Radiological Protection and Nuclear Emergency,Beijing 100088,China)
出处
《癌变．畸变．突变》
CAS
2020年第3期166-170,176,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金(81573081)。
