白及Aux/IAA基因的鉴定及分析

Identification and analysis on Aux/IAA genes of Bletilla striata

基于白及〔Bletilla striata(Thunb.ex A.Murray)Rchb.f.〕的全转录组数据,采用生物信息学方法对白及Aux/IAA基因进行鉴定和序列分析,对白及Aux/IAA蛋白的一级和二级结构、基序、保守结构域、GO功能分类和KEGG富集结果进行分析,并对其与拟南芥〔Arabidopsis thaliana(Linn.)Heynh.〕和铁皮石斛(Dendrobium officinale Kimura et Migo)的Aux/IAA蛋白进行系统发育分析。结果表明:在白及的全转录组中共筛选出5个Aux/IAA基因,分别命名为BsAux/IAA1至BsAux/IAA5,开放阅读框(ORF)长度为492~2739 bp。5个BsAux/IAA蛋白的氨基酸残基数为163~912,理论相对分子质量为18172.97~101442.89,理论等电点为pI 4.40至pI 11.51,亚细胞定位于细胞核中,二级结构以α-螺旋和无规则卷曲为主。5个BsAux/IAA蛋白共有DomainⅠ、DomainⅡ、DomainⅢ和DomainⅣ4个完整的保守结构域,依次对应保守基序motif9、motif3、motif2和motif1。5个BsAux/IAA蛋白的功能均包括细胞组分、生物学过程和分子功能3个大类,并参与植物激素信号转导通路。系统发育分析结果表明:5个BsAux/IAA蛋白均属于GroupⅠ亚家族,并进一步分成a和b 2个亚群,其中,BsAux/IAA3、BsAux/IAA4和BsAux/IAA5属于a亚群,BsAux/IAA1和BsAux/IAA2属于b亚群;并且,BsAux/IAA1、BsAux/IAA2、BsAux/IAA4和BsAux/IAA5与铁皮石斛Aux/IAA的亲缘关系较近。研究结果显示:白及Aux/IAA蛋白具有一定的保守性,可能在细胞核中发挥作用,并具有组织特异性、发育依赖性和非生物胁迫应答性。

Based on the whole transcriptome data of Bletilla striata(Thunb.ex A.Murray)Rchb.f.,identification and sequence analysis on Aux/IAA genes of B.striata were conducted by using bioinformatics methods,and primary and secondary structures,motif,conserved domain,GO function classification,and KEGG enrichment result of Aux/IAA proteins of B.striata were analyzed,meanwhile phylogenetic analysis on them with Aux/IAA proteins of Arabidopsis thaliana(Linn.)Heynh.and Dendrobium officinale Kimura et Migo were performed.The results show that 5 Aux/IAA genes are screened from the whole transcriptome of B.striata,named as BsAux/IAA1 to BsAux/IAA5,respectively,with an open reading frame(ORF)length of 492-2739 bp.The amino acid residue numbers of 5 BsAux/IAA proteins are 163-912,with theoretical relative molecular mass of 18172.97-101442.89 and theoretical isoelectric point of pI 4.40 to pI 11.51,their subcellular locations are in the nucleus,and their secondary structures are mainlyα-helix and random coil.There are 4 complete conserved domains of DomainⅠ,DomainⅡ,DomainⅢ,and DomainⅣin 5 BsAux/IAA proteins,corresponding to conserved motifs of motif9,motif3,motif2,and motif1 in order.The functions of 5 BsAux/IAA proteins all include 3 categories of cellular component,biological process,and molecular function,and are involved in signal transduction pathways of plant hormones.The phylogenetic analysis result shows that 5 BsAux/IAA proteins all belong to GroupⅠsubfamily,and can be divided into 2 subgroups of a and b,in which,BsAux/IAA3,BsAux/IAA4,and BsAux/IAA5 belong to a subgroup,while BsAux/IAA1 and BsAux/IAA2 do to b subgroup;in addition,there are close genetic relationships in BsAux/IAA1,BsAux/IAA2,BsAux/IAA4,and BsAux/IAA5 with Aux/IAA of D.officinale.It is suggested that there is a certain conservation in Aux/IAA proteins of B.striata,the proteins may play roles in nucleus,and possess tissue specificity,development dependence,and abiotic stress response.