基质中添加多孔改良剂对南方高丛蓝浆果品种‘蓝美1号’组培苗茎段瓶外生根的影响

Effects of substrate complemented with porous soil amendment on ex vitro rooting of stem segments of tissue culture seedlings of Vaccinium corymbosum‘Lanmei 1’

蓝浆果(Vaccinium spp.)组培苗在瓶内生根较慢且生根率较低[1],并且瓶内生根苗的茎、叶柄和根系在移栽过程中易受损伤,影响移栽成活率[2],采用瓶外生根可以降低育苗成本、提高工作效率[2-4]。目前,苔藓是蓝浆果组培苗插条扦插常用基质,但在扦插过程中,组培苗插条基部易腐烂,且夏季其生根率更低。

The effects of substrates complemented with different particle sizes and proportions of porous soil amendments on ex vitro rooting of stem segments of tissue culture seedlings of Vaccinium corymbosum‘Lanmei 1’were studied.The results show that all rooting indexes of stem segments of tissue culture seedlings of‘Lanmei 1’in B2〔V(moss,M)∶V(porous soil amendments with particle size of 1-3 mm,PM1)=4∶1〕substrate are the best,in which,rooting rate and comprehensive rooting quality at the 14th day after cutting are significantly higher than those in CK2(M)substrate.All rooting indexes of stem segments of tissue culture seedlings of‘Lanmei 1’in B1〔V(M)∶V(PM1)=9∶1〕substrate are also relatively good,and are not significantly different from those in B2 substrate.If considering economic cost,B1 substrate is better.