蠕虫床污泥减量对A^2O-MBR系统中细菌特性的影响

Effects of worm predation and sludge reduction on bacterial characteristics of an anaerobic-anoxic-oxic-membrane bioreactor(A^2O-MBR)

蠕虫捕食是实现污泥减量最有前景的技术之一,具有高效和环保的优点。为了考察蠕虫捕食对污水处理系统微生物特性的影响,建立了厌氧-缺氧-好氧-膜生物反应器(A^2O-MBR)与蠕虫床耦合系统,并以传统的A^2O-MBR、A^2O-MBR-空白蠕虫床耦合系统为对照,3组系统同时稳定运行120 d,采用16S rRNA高通量测序对反应器的微生物特性进行对比分析研究。结果表明,A^2O-MBR-蠕虫床系统微生物丰度变大,多样性降低,有效促进了优势菌的富集;耦合系统中脱氮除磷功能菌得到强化,其中反硝化除磷菌比例达12.71%,使脱氮与除磷过程相互协同,蠕虫床的耦合提高了A^2O-MBR系统脱氮除磷效能。以上研究结果为蠕虫捕食技术在污泥减量与污水处理中的工程应用提供了一条有效途径。

Worm predation is one of the most promising sludge reduction technologies,with the advantages of high efficiency and environmental friendliness.In order to investigate the effects of worm predation on the bacterial characteristics of the wastewater treatment process,a combined system of an anaerobic-anoxic-oxicmembrane bioreactor(A^2O-MBR)and worm reactor was established.A conventional A^2O-MBR and a combined system of A^2O-MBR and blank worm reactor were established as two control systems.The bacterial characteristics of three systems were analyzed by high-throughput sequencing of the 16S rRNA gene after 120 days stable operation.The results showed that the microbe richness increased while the diversity decreased in the A^2O-MBR-worm reactor system,which promoted the enrichment of the dominant bacteria.In the combined system,the functional bacteria for nitrogen and phosphorus removal were enriched,of which the abundance of denitrifying phosphorus removal bacteria reached 12.71%,and the synergistic removal of nitrogen and phosphorus occurred.The combination of worm predation improved the removal efficiencies of nitrogen and phosphorus in A^2O-MBR system.This study provided an efficient approach for practical application of worm predation for sludge reduction and wastewater treatment.