血清miR-711、miR-203对阿尔茨海默病的诊断效能评估

Evaluation of Diagnostic Efficacy of Serum miR-711 and miR-203 in Alzheimer′s Disease

目的探讨miR-711、miR-203在阿尔茨海默病(AD)患者血清中的表达水平及其临床意义。方法收集18例正常对照(NC)、18例遗忘型轻度认知障碍(aMCI)和18例阿尔茨海默病(ADD)患者的血清学标本,采用荧光定量PCR技术检测血清中miR-711和miR-203的表达水平,分析两者与MoCA评分的相关性,并应用ROC曲线和Logistic回归模型评价血清miR-711及miR-203在aMCI和ADD中的诊断价值。结果血清miR-711和miR-203在ADD患者中表达均明显增高(P=0.000);aMCI组血清miR-711表达与正常对照组比较有明显增高(P<0.01)、与ADD组比较差异无统计学意义(P>0.05);aMCI组血清miR-203表达与ADD组比较是减低的(P<0.01)、与对照组比较差异无统计学意义(P>0.05)。相关性分析显示血清miR-711和miR-203表达与MoCA评分均呈负相关(r=-0.52,P=0.000;r=-0.49,P=0.000)。血清miR-711用于诊断aMCI时,AUC为0.812;血清miR-203用于区分aMCI及ADD时,AUC为0.718;血清miR-711及miR-203用于诊断ADD时,AUC分别为0.875、0.889,两者联合检测ADD时,AUC为0.948。结论血清miR-711和miR-203可能成为诊断AD的潜在生物学标志物。

Objective To investigate the expression levels of miR-711 and miR-203 in peripheral blood serum of patients with Alzheimer′s disease(AD)and discuss their clinical significance.Methods Eighteen normal controls(NC),eighteen patients with amnestic mild cognitive impairment(aMCI)and eighteen patients with Alzheimer′s disease dementia(ADD)were enrolled in this study.The expression levels of miR-711 and miR-203 in peripheral blood serum were determined by real-time fluorescent quantitative PCR,respectively,the correlation between their expression levels and the scores of MoCA was analyzed.In addition,the diagnostic value of serum miR-711 and miR-203 in aMCI and ADD was evaluated by receiver operating characteristic curve(ROC)and Logistic regression model.Results The expression levels of serum miR-711 and miR-203 in ADD group were significantly higher than that in NC group(P=0.000).The expression levels of serum miR-711 in aMCI group were significantly higher than that in NC group(P<0.01),but there was no significance compared with ADD group(P>0.05).The expression levels of serum miR-203 in aMCI group were lower than that in ADD group(P<0.01),while there was no significant compared with the NC group(P>0.05).Correlation analysis showed that the expression levels of miR-711 and miR-203 in serum were negative to the MoCA score(r=-0.52,P=0.000;r=-0.49,P=0.000).When serum miR711 is used for diagnosis of aMCI,AUC is 0.812.When the serum miR-203 was used to distinguish aMCI and ADD,AUC was 0.718.When the serum miR-711 and miR-203 were respectively used for the diagnosis of ADD,AUC was 0.875 and 0.889.When miR-711 and miR-203 are combined to diagnose ADD,the AUC was 0.948.Conclusion Serum miR-711 and miR-203 may be potential biological markers for diagnosis of AD.