辣椒根际促生菌的分离筛选及抗病促生特性研究

Isolation and Screening of Plant Growth-promoting Rhizobacteria in Pepper and Their Disease-resistant Growth-promoting Characteristics

获得辣椒根际促生菌(Plant growth promoting rhizobacteria,PGPR)并探究其抗病促生特性。采用固氮、无机磷和有机磷培养基从江苏省徐州市采集的辣椒根际土壤中分离筛选根际促生菌株(PGPR),通过形态特征及16S rDNA序列分析进行菌株鉴定,对菌株的固氮、解磷、分泌3-吲哚乙酸(IAA)能力及对4种辣椒病害病原菌抗病能力进行探究。得到13株辣椒PGPR菌株,经鉴定分别属于Bacillus、Pseudomonas、Lelliottia、Siccibacter、Achromobacter、Microbacterium和Paenibacillus;13株PGPR菌株均有固氮功能;其中7株可解有机磷,分别属于Lelliottia、Bacillus、Siccibacter、Microbacterium、Paenibacillus;5株可解无机磷,分别属于Lelliottia、Bacillus、Siccibacter、Pseudomonas;3株具有分泌IAA能力,分别属于Lelliottia、Siccibacter、Bacillus;5株具有抗病能力,分别属于Bacillus、Lelliottia、Siccibacter。辣椒根际土壤含有在农业生产上具有潜在的应用价值的多功能根际促生菌。

In order to obtain plant growth-promoting rhizobacteria(PGPR)of pepper and to explore their disease-resistant and growth-promoting characteristics,the PGPR were isolated and screened from pepper rhizosphere soil in Xuzhou city of Jiangsu province,using nitrogen fixation,inorganic phosphorus and organic phosphorus media.The strains were identified by morphological characteristics and 16SrDNA.Their abilities of fixing nitrogen,releasing phosphorus,secreting IAA and resistances to 4 pepper pathogenic diseases were investigated.The results showed that 13 PGPR strains of pepper were obtained and identified as Bacillus,Pseudomonas,Lelliottia,Siccibacter,Achromobacter,Microbacterium and Paenibacillus.All of them had function of fixing nitrogen,7 of them dissolved organic phosphorus,belonging to Lelliottia,Bacillus,Siccibacter,Microbacterium and Paenibacillus;5 dissolved solubilized inorganic phosphorus,belonging to Lelliottia,Bacillus,Siccibacter,and Pseudomonas;3 presented the ability of secreting IAA,belonging to Lelliottia,Siccibacter,Bacillus;and 5 demonstrated the resistances to diseases,belonging to Bacillus,Lelliottia,and Siccibacter.Pepper rhizosphere soil contains multi-functional PGPR,which has potential application value in agricultural production.