摘要
人绒毛膜滋养层细胞(HTR8/SVneo)是胎盘建立血液循环的重要组成部分,过氧化物酶体增殖物激活受体α(PPARα)是调节脂代谢的关键转录因子亚型,本研究旨在研究PPARα对人绒毛滋养层细胞功能的影响。将构建的PPARα表达载体和PPARα小干扰RNA分别转染HTR8/SVneo细胞,检测细胞功能的变化。本实验采取EdU法和MTT法检测细胞增殖,流式细胞术检测细胞凋亡,transwell小室法检测细胞迁移和浸润能力。结果显示,PPARα过表达能抑制滋养层细胞增殖、迁移和浸润能力,促进细胞凋亡能力;敲低PPARα能促进细胞的增殖、迁移和浸润能力,抑制细胞凋亡能力。PPARα表达水平与其对细胞生长和迁移的影响负相关。
HTR8/SVneo cells are an important component of the placenta to establish blood circulation.Peroxisome proliferator-activated receptor alpha(PPARα)is a key factor regulating lipid metabolism.This study aims to clarify the influence of PPARαon human trophoblast cell function.The constructed PPARαexpression vector and PPARαsmall interfering RNA were transfected into HTR8/SVneo cells,respectively,to detect changes in cell function.EdU method and MTT method were used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,and transwell chamber method was used to detect cell migration and infiltration capacity.The number of samples in each group is not less than three,and the experiment is repeated three times.The experimental data were statistically analyzed using GraphPad Prism 6 software.Data were processed using t test and analysis of variance.P<0.05 was considered statistically significant.The results showed that PPARαoverexpression inhibited the proliferation,migration and infiltration of trophoblast cells and promoted apoptosis(P<0.05,Fig.2 a,3 a,c,4 a,b,5 a,b,6 a,b);knockdown of PPARαpromoted cell proliferation,migration and infiltration,and inhibited apoptosis(P<0.05,Fig.2 b,3 b,d.4 c,d,5 c,d,6 c,d).The level of PPARαexpression is negatively correlated with its effect on cell growth and migration.
作者
管纯一
马旭
夏红飞
GUAN Chun-Yi;MA Xu;XIA Hong-Fei(National Research Institute for Family Planning,Beijing 100081;Peking Union Medical College,Beijing 100005,China)
出处
《动物学杂志》
CAS
CSCD
北大核心
2020年第2期213-221,共9页
Chinese Journal of Zoology
基金
国家自然科学基金项目(No.81771590)
中央国家机关基础科研业务费(No.2018GJZ04)。