摘要
目的:比较南天竹的不同药用部位对抗肿瘤药三氧化二砷的减毒作用,为南天竹的深度开发应用提供科学依据。方法:采用大鼠慢性三氧化二砷中毒模型,将56只SD大鼠随机分为空白组;模型组(信石40 mg·kg^-1);2,3-二巯基丙磺酸钠组(2,3-二巯基丙磺酸钠25 mg·kg^-1+信石40 mg·kg^-1);南天竹根组(南天竹根20 g·kg^-1+信石40 mg·kg^-1);南天竹茎组(南天竹茎20 g·kg^-1+信石40 mg·kg^-1);南天竹叶组(南天竹叶20 g·kg^-1+信石40 mg·kg^-1);南天竹果实组(南天竹果实20 g·kg^-1+信石40 mg·kg^-1)。连续灌胃给药10 d。末次给药后,收集24 h内尿液,手术后收集血清、肾组织及肝组织样本,测定血肌酐(serum creatinine,SCr)及尿肌酐(urine creatinine,UCr)水平,计算内生肌酐清除率(creatinine clearance,CCr),同时检测各组大鼠肝脏、肾脏中丙二醛(malondialdehyde,MDA),总超氧化物歧化酶(superoxide dismutase,SOD),过氧化氢酶(catalase,CAT)水平,肾脏及肝脏苏木精-伊红染色后显微镜下观察组织病理形态学变化。结果:与模型组比较,南天竹根、茎、果实联合给药组大鼠体质量显著增加(P<0.01),肾脏系数显著降低(P<0.01),大鼠UCr和CCr水平显著升高(P<0.01),肾组织中MDA含量显著下降(P<0.01),肝组织中MDA水平明显降低(P<0.05),肝、肾组织中SOD和CAT活性显著升高(P<0.01),大鼠肝、肾损害病理损伤减轻;南天竹叶联合给药组除肝脏中SOD活性与模型组无明显差异外,其余指标与以上3组变化一致。结论:南天竹根、茎、果实对三氧化二砷氧化应激所致肝、肾毒性有显著的保护作用,南天竹叶效果稍差。
Objective:To compare the effect of different medicinal parts of Nandina domestica in reducing toxicity of anti-tumor drug arsenic trioxide,so as to provide the scientific basis for its further development and application.Method:Chronic arsenic trioxide poisoning model was used in this paper.Totally 56 SD rats were randomly divided into normal group,model group(arsenolite 40 mg·kg^-1),sodium 2,3-dimercapto-1-propanesulfonate group(sodium 2,3-dimercapto-1-propanesulfonate 25 mg·kg^-1+arsenolite 40 mg·kg^-1),Nandinae Radix group(Nandinae Radix 20 g·kg^-1+arsenolite 40 mg·kg^-1),Nandinae Caulis group(Nandinae Caulis 20 mg·kg^-1+arsenolite 40 mg·kg^-1),Nandinae Folium group(Nandinae Folium 20 g·kg^-1+arsenolite 40 mg·kg^-1),and Nandinae Fructus group(Nandinae Fructus 20 g·kg^-1+arsenolite 40 mg·kg^-1).The intragastric administration lasted for 10 days.After the last administration,urine was collected within 24 hours,serum,kidney and liver tissue samples were collected after operation,and serum creatinine(SCr)and urine creatinine(UCr)levels were measured,in order to calculate endogenous creatinine clearance rate(CCr).At the same time,the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and catalase(CAT)in liver and kidney of rats in each group were detected.Some kidneys and livers were fixed with formaldehyde,and the histopathological changes were observed under microscope after hematoxylin-eosin staining.Result:Compared with the model group,the rats in combination group of Nandinae Radix,Nandinae Caulis,Nandinae Fructus have a heavier body mass(P<0.01),the kidney coefficient was lower(P<0.01),the levels of UCr and CCr were significantly increased(P<0.01),the content of MDA in renal tissue was decreased significantly(P<0.01),the level of MDA in liver tissue was decreased(P<0.05),whereas the activities of SOD and CAT were significantly increased(P<0.01),the pathological damage of liver and kidney was alleviated.There was no significant difference in the activity of SOD in the liver between the Nandinae Folium combination group and the model group,but the changes of the other indexes were consistent with those of the above three groups.Conclusion:Nandinae Radix,Nandinae Caulis,Nandinae Fructus have significant protective effects on liver and kidney toxicity induced by arsenic trioxide oxidative stress,and Nandinae Folium was the least effective among them.
作者
孙菡
刘婧
吴志瑰
付小梅
彭财英
王芳
杨超
刘世欣
SUN Han;LIU Jing;WU Zhi-gui;FU Xiao-mei;PENG Cai-ying;WANG Fang;YANG Chao;LIU Shi-xin(School of Pharmacy,Jiangxi University of Traditional Chinese Medicine(TCM),Nanchang 330004,China;FAN Cui-sheng Studio of National Famous TCM,Nanchang 330006,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第9期136-142,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81760721)
江西中医药大学研究生创新专项(JZYC18S10)
江西省研究生优质课程(中药鉴定学)
江西中医药大学研究生核心课程及课程案例库(2015YJS012,2015YJSZ008)
江西省中药学一流学科专项科研基金项目(JXSYLXK-ZHYAO026)
2018年全国中药特色技术传承人才培训项目(国中医药人教函[2018]204号)。
关键词
南天竹
肝保护
肾保护
氧化损伤
三氧化二砷
Nandina domestica
liver protection
kidney protection
oxidative damage
arsenic trioxide