基于UPLC-Q-TOF-MS/MS技术快速鉴定交趾黄檀心材中化学成分

Rapid Identification and Analysis of Chemical Components in Heartwood of Dalbergia cochinchinensis by UPLC-Q-TOF-MS/MS

目的:应用超高效液相色谱-四极杆-飞行时间高分辨率质谱(UPLC-Q-TOF-MS/MS)技术快速分析与鉴定黄檀属植物交趾黄檀心材甲醇提取物中的化学成分。方法:采用UPLC RRHD SB-C18色谱柱(3.0 mm×100 mm,1.8μm),流速0.3 mL·min^-1,进样量1μL,流动相0.1%甲酸水溶液(A)-乙腈(B)梯度洗脱(0~0.01 min,5%B;0.01~2 min,5%~22%B;2~28 min,22%~35%B;28~45 min,35%~44%B;45~55 min,44%~100%B;55~57 min,100%B;57~57.10 min,100%~5%B);选择电喷雾离子源,负离子模式采集数据,采集范围m/z 100~1500。结果:从交趾黄檀心材的甲醇提取物中初步鉴定了101个化学成分,包括22个黄酮类,34个异黄酮类,15个新黄酮类,18个其他类型黄酮和12个其他类成分。结论:UPLC-Q-TOF-MS/MS技术方法可快捷、准确、较全面地鉴定交趾黄檀心材中的化学成分,交趾黄檀心材中主要化学成分为异黄酮类、黄酮类和新黄酮类成分,对揭示其内在物质基础具有重要意义,也为其作为潜在的中药新资源开发提供了实验依据。

Objective:To rapidly identify and analysis the chemical constituents in the methanol extract of heartwood of Dalbergia cochinchinensis by ultra high performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry(UPLC-Q-TOF-MS/MS).Method:UPLC RRHD SB-C18 column(3.0 mm×100 mm,1.8μm)was used for chromatographic separation with acetonitrile-0.1%formic acid solution as the mobile phase for gradient elution(0-0.01 min,5%B;0.01-2 min,5%-22%B;2-28 min,22%-35%B;28-45 min,35%-44%B;45-55 min,44%-100%B;55-57 min,100%B;57-57.10 min,100%~5%B)at a flow rate of 0.3 mL·min^-1.The analytes were determined in negative ion mode with electrospray ionization(ESI)and data collection range of m/z 100-1500.Result:A total of 101 chemical constituents were identified,including 22 flavonoids,34 isoflavones,15 neoflavonoids,18 other flavonoids and 12 other components.Conclusion:UPLC-Q-TOF-MS/MS technique can quickly,accurately and comprehensively identify the chemical constituents in the heartwood of D.cochinchinensis.Isoflavones,flavonoids and neoflavonoids are the main chemical constituents in the heartwood of D.cochinchinensis,which is of great significance to reveal its internal material basis and provides experimental basis for this plant to be developed as a potential new resource of traditional Chinese medicine.