DNA步行器的构建及其在生命分析的应用

Construction of DNA walker and its application in bioanalytical analysis

DNA具有独特的序列依赖性和可编辑性,依赖于此,大量的DNA纳米器件被开发应用,如镊子、齿轮、起重机。在这些器件中,DNA步行器能够依靠链置换、酶剪切、光诱导等行走动力沿着特定的一维、二维、三维的轨道运动,展现出独特的方向性、动态性,被广泛应用于生命分析化学领域。鉴于DNA步行器广阔的应用前景,该综述从步行器的构造、设计原则、行走动力,和其在生命分析应用的角度出发,详细进行了阐述。

DNA with sequence specificity and excellent programmability,has been designed to various functional DNA devices,such as tweezers,gears,cranes and so on.Especially,DNA walkers are a unique one of those dynamic DNA devices,which could move possessively along designated one-,two-,or three-dimensional tracks by the walking power of strand-displacement,enzyme cleavage or photoinduction,showing a unique mechanical motion,dynamic interaction,and paving a new avenue for bioanalytical analysis.In view of the wide application prospect of DNA walker,this review elaborates in detail from the perspectives of the structure,design principle,walking power and application of bioanalytical analysis of the walker.