甘薯盐胁迫响应基因IbMYB3的表达特征及生物信息学分析

Expression Patterns and Bioinformatic Analyses of Salt Stress Responsive Gene IbMYB3 in Ipomoea batatas

MYB转录因子具有多种生物学功能,在植物响应生物和非生物胁迫中发挥重要作用。该文从盐胁迫后的甘薯(Ipomoea batatas)水培苗转录组数据(RNA-seq)中筛选出2个受盐胁迫显著上调表达的MYB基因,分别命名为IbMYB3和IbMYB4。多种非生物胁迫和植物生长物质处理下的基因表达分析显示,IbMYB3受逆境诱导显著上调表达,暗示其可能参与甘薯非生物胁迫响应。生物信息学分析表明,IbMYB3开放阅读框长度为1059 bp,编码353个氨基酸残基,蛋白分子量为39.41 kDa,理论等电点(PI)为5.26,为酸性带负电的亲水性蛋白。亚细胞定位结果表明,IbMYB3蛋白定位于细胞核,具有较强的转录激活活性。上述结果表明,IbMYB3转录因子可能在甘薯非生物胁迫响应过程中发挥重要调控作用,研究结果为进一步探明IbMYB3基因的功能奠定了基础。

MYB transcription factors have multiple biological functions and play important roles in mediating plant responses to biotic and abiotic stresses.In this paper,two MYB genes,named IbMYB3 and IbMYB4,which were significantly induced by salt stress,were screened from RNA-seq data of salt-stressed sweetpotato(Ipomoea batatas)plantlets.Gene expression analysis showed that the expression of IbMYB3 was significantly up-regulated by various abiotic stresses and plant growth substances treatments,suggesting that IbMYB3 might be involved in the abiotic stress responses of sweetpotato.Further bioinformatic analysis showed that the open reading frame of IbMYB3 is 1059 bp in length,encoding 353 amino acids,with a predicted molecular weight of 39.41 kDa and the theoretical isoelectric point(PI)of 5.26,which is an acidic negatively charged hydrophilic protein.Subcellular localization showed that the IbMYB3 protein localizes to the nucleus,and has strong transcriptional activation activity.Taken together,these results demonstrated that the IbMYB3 transcription factor might play an important role in regulating the abiotic stress responses of sweetpotato.This study thus lays the foundation for further illustration of IbMYB3 function.