中国部分地区柯萨奇病毒B组5型分子流行病学研究

Molecular epidemiological analysis on coxsackievirus B5 in certain areas of China

目的 研究中国部分地区柯萨奇病毒B组5型(coxsackievirus B5, CV-B5)分离毒株基因特征和遗传进化规律。方法 利用MEGA6.0软件对GenBank核苷酸数据库收录的分离于中国11个省市CV-B5分离毒株全长VP1区基因序列进行分析,绘制系统进化树,测算分离毒株核苷酸和氨基酸同源性,并计算核苷酸分子进化速率。结果 纳入中国11个省市CV-B5分离毒株合计189株,主要为基因型C,占分离毒株总数的90.5%。与基因型A相比,中国11个省市CV-B5分离毒株VP1区核苷酸和氨基酸同源性为79.8%~82.8%和92.6%~97.9%,收录中国地区CV-B5分离毒株间核苷酸和氨基酸同源性为80.3%~100.0%和91.5%~100.0%。基因型C分离毒株核苷酸进化速率为4×10-3/(位点/年)。结论 中国部分地区CV-B5分离毒株潜在优势流行株为基因型C,又以基因亚型C1和C2共循环流行。

Objective To study the molecular epidemiology and genetic variation of coxsackievirus B5(CV-B5)in certain areas in China.Methods MEGA 6.0 software was used to analyze the complete VP1 region of CV-B5 isolated strains from certain areas of China by retrieving the GenBank nucleotide database.Besides,the phylogenetic tree was constructed,the homology of nucleotide and amino acids were calculated and the rate of evolution was estimated.Results A total of 189 Chinese CV-B5 isolated strains were included in this study.Most of Chinese CV-B5 isolated strains belonged to genotype C,accounted for 90.5%.Compared with the genotype A,the homology of nucleotide sequences and amino acid sequences of complete VP1 region of 189 Chinese isolated strains were 79.8%-82.8%and 92.6%-97.9%,respectively;moreover,the nucleotide and the amino acid homology of 189 Chinese CV-B5 isolated strains among themselves ranged from 80.3%to 100.0%and ranged from 91.5%to 100.0%,respectively.The estimated rate of evolution of the CV-B5 was 4×10-3 substitutions/site/year.Conclusions The majority of CV-B5 isolated strains belonged to genotype C,and subgenotype C1 and C2 were co-circulating together in certain areas of China.