摘要
对威宁红花油茶(Camellia weiningensis)的叶片高通量测序数据进行叶绿体全基因组的组装和注释分析。其叶绿体全基因组长为156490 bp,包含典型的四分体结构,序列已登录GenBank(MK820035)。叶绿体全基因组比较分析表明,与其他山茶属物种一样,其结构与基因排序均保守,但在基因组的反向重复区边界处表现出明显区别。简单重复序列(SSR)分析表明,全基因组仅包含51个单核苷酸重复的SSR,且仅为A/T重复类型。系统发育分析表明,威宁红花油茶与腾冲红花油茶亲缘关系最近,但其所处分支包含的4个山茶属物种的分组与传统分类不一致。
The whole chloroplast genome of Camellia weiningensis was assembled,annotated and analyzed.The results showed that the complete length of the chloroplast genome was 156490 bp,including typical tetrad structure,and the sequence was deposited in GenBank(MK820035).Comparative analysis of the complete genome of chloroplast showed that,like other Camellia species,the structure and gene order are conservative,but there are still significant differences at the boundaries of the inverted repeat regions of the genome.Simple sequence repeats(SSR)analysis showed that the whole genome contained only fifty-one mononucleotide repeats,and they were only A/T repeat type.Phylogenetic analysis based on the whole chloroplast genome showed that C.weiningensis was most related to C.reticulata,but clusters of the four Camellia species were not consistent with the traditional section classification.
作者
李倩
郭其强
高超
李慧娥
LI Qian;GUO Qiqiang;GAO Chao;LI Huie(Department of horticulture,College of Agriculture,Guizhou University,Guiyang 550025,China;Institute for Forest Resources&Environment of Guizhou Province,Guizhou University,Guiyang 550025,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2020年第4期779-787,共9页
Acta Horticulturae Sinica
基金
贵州省生物学一流学科建设项目(GNYL[2017]009)
国家自然科学基金项目(31800516)
贵州省科技计划项目(黔科合支撑[2019]2310,2314)。