陕西杨凌野菊花挥发性成分固相微萃取气相色谱-质谱分析

Analysis of the volatile constituents of flos chrysanthemi indici in Yangling demonstration zone of Shaanxi by SPME/GC/MS

目的分析野菊花挥发性成分的主要化学成分。方法采用固相微萃取法提取、富集野菊花中的挥发性成分,采用气相色谱-质谱法对其化学成分进行鉴定,用面积归一化法计算各组分相对百分含量。结果共鉴定出50种化学成分,占挥发性成分含量的97.17%。野菊花挥发性成分主要为桉油精(24.72%)、α-香叶烯(15.78%)、β-金合欢烯(10.44%)、樟脑(10.05%)、α-衣兰油烯(4.81%)、α-水芹烯(4.43%)、α-蒎烯(4.28%)、莰烯(3.61%)、乙酸龙脑酯(2.56%)、侧柏烯(1.59%)、雪松烯(1.47%)等。结论固相微萃取技术可用于野菊花挥发性成分分析,并提高其灵敏度与准确性。

Objective To analyze the chemical constituents of the volatile constituents of Flos chrysanthemi indici.Methods The volatile constituents in Flos chrysanthemi indici was extracted by the method of Solid phase microextraction(SPME).The components of the volatile constituents were separated and identified by gas chromatography-mass spectrometry(GC-MS).The relative content of each component was determined by area normalization.Results Fifty different compounds were isolated and identified,accounting for 97.17%of the total volatile constituents.The main components in volatile constituents of Flos chrysanthemi indici were eucalyptol(24.72%),á-Myrcene(15.78%),1,6,10-Dodecatriene,7,11-dimethyl-3-methylene-,(Z)-(10.44%),Camphor(10.05%),Naphthalene,1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-,(1à,4aà,8aà)-(4.81%),á-Phellandrene(4.43%),1R-à-Pinene(4.28%),Bornyl acetate(2.56%),Bicyclo[3.1.0]hexane,4-methylene-1-(1-methylethyl)-(1.59%),1H-Benzocycloheptene,2,4a,5,6,7,8-hexahydro-3,5,5,9-tetramethyl-,(R)-(1.47%).Conclusions The SPME technology can be used for volatile component analysis of wild chysanthemum,and it can help to improve the sensitivity and accuracy.