超临界CO2萃取不同产地云南松松针挥发油及其GC-MS分析

Supercritical CO2 Extraction of Volatile Oil in Pine Needle of Pinus yunnanensis from Different Habitats and Analysis by GC-MS

目的:分析比较不同产地云南松松针的挥发油成分。方法:采用超临界CO2萃取法(SFE-CO2)提取12个不同产地的云南松松针挥发油,通过气相色谱-质谱法(GC-MS)进行成分分析,峰面积归一化法计算各组分的相对百分含量,并利用SPSS 20.0软件对结果进行主成分分析。结果:从12个不同产地的云南松松针挥发油成分中共分离鉴定出137种化合物,其中1号样品鉴别出57种,2号样品鉴别出57种,3号样品鉴别出54种,4号样品鉴别出48种,5号样品鉴别出58种,6号样品鉴别出60种,7号样品鉴别出53种,8号样品鉴别出55种,9号样品鉴别出56种,10号样品鉴别出58种,11号样品鉴别出54种,12号样品鉴别出50种,挥发油成分由烃、酸、醇、酯、醛、酮、酚、醚8类化合物组成,其中共有成分有11种,包括α-蒎烯,β-蒎烯,β-石竹烯,7,10,13-十六碳三烯酸,棕榈油酸甲酯,棕榈酸,邻苯二甲酸二丁酯,植物醇,硬脂酸,山嵛酸,木焦油酸。结论:该研究采用超临界CO2萃取(SFE-CO2)法提取不同产地的云南松松针挥发油,发现云南松松针挥发油成分主要是烃类、酸类、酯类、醇类化合物,不同产地的云南松松针挥发油成分的含量及种类相差较大。为云南松松针资源进一步开发利用奠定基础。

Objective:To analyze and compare the volatile oil components of pine needle of Pinus yunnanensis from different habitats.Method:The volatile oils of pine needle of P.yunnanensis from 12 different habitats were extracted by supercritical CO2 extraction(SFE-CO2),the components were analyzed by gas chromatography-mass spectrometry(GC-MS),and relative percentage of each component was determined by peak area normalization method,and SPSS 20.0 software was used for principal component analysis.Result:The 137 compounds were isolated from the volatile oil of pine needle of P.yunnanensis from 12 different producing areas,with 1 sample identified 57 species,2 samples identified 57 species,3 samples identified 54 species,4 samples identified 48 species,5 samples to identify 58 species,6 samples identified 60 species,7 samples identified 53 species,8 samples identified 55 species,9 samples identified 56 species,10 samples to identify 58 species,11 samples identified 54 species and 12 samples to identify 50 species,composed of hydrocarbon,acid,alcohol,ester,aldehyde,ketone,phenol,ether 8 compounds,including 11 common composition including alpha pinene,beta pinene,beta caryophyllene,7C,10C,16C-hexadecatrienoic acid and palmitic acid methyl ester,palmitic acid,phthalic acid dibutyl do acid,alcohol,stearic acid,mountain,wood tar acid. Conclusion: In this study,supercritical CO2 extraction(SFE-CO2)was used to extract the volatile oil ofpine needle of P. yunnanensis from different habitats. It was found that the volatile oil of pine needle of P.yunnanensis was mainly composed of hydrocarbons,acids,esters and alcohols. And the content and species ofvolatile oil of pine needle of P. yunnanensis from different producing areas differ greatly which lay a foundationfor the further development and utilization of pine needle of P. yunnanensis resources.