一种微量样品快速测定比色法在水环境监测中的应用研究

Application of a fast colorimetric method for determination of micro-samples in water environment monitoring

分光光度法在我国水环境监测领域应用广泛,但在分析批量样品时需投入大量人力物力,成本较高。利用酶标仪微量比色法,提出了一种对微量、批量样品的快速测定方法。以水中氨氮检测为例,确定了该方法的最佳参数和条件,并与传统的分光光度法进行比较。酶标仪微量比色法与分光光度法在线性范围、检出限、最低检出限、灵敏度等分析参数上没有明显差异。通过对实验条件的优化,酶标仪微量比色法测定氨氮仅需样品体积200μL,纳氏试剂4μL,1 min内可以完成96个样品的测定,弥补了分光光度法在批量操作上的不足,减少了实验废水的排放,使分析检测工作快速、准确和省时。

Spectrophotometry is widely used in water environment monitoring in China,but the analysis of batch samples is costly and needs much manpower and material resources.A rapid method to determine a batch of trace samples was established by using microplate reader.The concentration of ammonia nitrogen in water was measured by the microplate colorimetric method and the optimal parameters and conditions were determined.Compared with the traditional spectrophotometry,there are no significant differences in linear range,detection limit,minimum detection limit,sensitivity and other analytical parameters.The microplate colorimetric method requires only 200μL of the sample and 4μL of Nessler's reagent to determine the ammonia nitrogen and takes only one minute to complete the determination of 96 samples.It makes up for the deficiency of spectrophotometry in batch operation and can reduce the release of experimental wastewater.The microplate colorimetric method can make the analysis fast,accurate and time-saving.