酚酸类化感自毒物质对枸杞种子萌发的抑制作用

Allelopathic inhibition of phenolic acids on germination of Wolfberry(Lycium barbarum Linn.)

酚酸类化感自毒物质是导致农作物连作障碍的重要诱因之一。枸杞作为多年生木本植物,连作障碍突出,成因复杂。为揭示酚酸类物质对枸杞的化感自毒作用,研究了22种常见酚酸对枸杞种子萌发的抑制作用,并运用比较分子场分析方法(CoMFA)进行构效关系研究。结果发现,酚酸化合物对枸杞种子萌发作用的半抑制浓度IC50范围为39.94—115.97 mg/L;CoMFA结果表明,运用立体场和静电场能较好解释该类化合物对枸杞种子萌发的化感自毒特征,苯环1/2/3/4位具有大体积、1位具有强负电性取代基的酚酸化合物,对枸杞种子萌发具有较强抑制作用。研究结果可为客观评价酚酸类化合物对枸杞的化感自毒作用提供基础数据支撑。

Phenolic acid allelochemicals are some of the most important agents to cause continuous cropping obstacles.As a perennial woody plant,the continuous cropping obstacles of Wolfberry(Lycium barbarum Linn.)are serious,and their contributed factors are complicated.To determine the effects of phenolic acids on Wolfberry,the allelopathic inhibition of 22 phenolic acids on the germination of wolfberry was quantified,and comparative molecular field(CoMFA)analysis was used to elucidate the relationship between allelopathic activities and structural characteristics.The results showed that half maximal inhibitory concentrations(IC50)were in 39.94—115.97 mg/L.The CoMFA indicated that the allelopathic activities increased when substituents had large volumes at the 1/2/3/4—positions of the benzene ring,and/or a negative charge at the 1-position of the benzene ring.These results provide basic data for evaluating the allelopathic effects of phenolic acids on Wolfberry.