EGCG及其络合物EGCG-Cu的消毒效果研究

Disinfection effect of EGCG and EGCG-Cu complexes

以微生物指标中代表性细菌大肠杆菌作为研究对象,探究EGCG及其络合物EGCG-Cu的抑菌活性及其影响因素。结果表明,EGCG-Cu与EGCG的最小抑菌浓度(MIC)分别为200 mg/L和1000 mg/L,EGCG-Cu络合物的抑菌能力优于EGCG。分析初始浓度和反应时间对EGCG-Cu和EGCG抑菌效果的影响表明,与EGCG相比,EGCG-Cu在较低浓度的抑菌效果更明显,但EGCG和EGCG-Cu达到较稳定抑菌率的反应时间均是6 h。通过测定大肠杆菌胞内活性氧簇(ROS)得出,EGCG-Cu诱导的氧化应激效应是其具有优异杀菌性的主要原因。综上表明,EGCG与金属离子络合可能是改进EGCG消毒效果的有效途径。

Taking E.coli as the research object,which is the most representative bacteria among the microbiological indicators.The antibacterial activity of EGCG and EGCG-Cu and their influencing factors were explored.The minimum inhibitory concentration(MIC)of EGCG-Cu and EGCG were 200~1000 mg/L respectively,and the bacteriostatic ability of the EGCG-Cu compleXwas found to be better than EGCG.The antibacterial effect of initial concentration and reaction time on EGCG-Cu and EGCG were analyzed.It was found that compared with EGCG,EGCG-Cu had more obvious antibacterial effect at lower concentration.However,the reaction time for EGCG and EGCG-Cu to achieve a stable bacteriostatic rate was 6 h.The reason for the excellent bactericidal ability of EGCG-Cu compleXwas investigated by measuring reactive oxygen species(ROS)in E.coli.The results indicated that EGCG-Cu induced oxidative stress,which was the main reason for its excellent bactericidal properties.In summary,the complexation of EGCG with metal ions may be an effective way to improve the disinfection of EGCG.