PK15细胞在不同培养条件下的生长情况

Growth of PK15 cells in different culture conditions

PK15细胞在多种兽用疫苗的研究和生产中被广泛培养。在生产中培养生长状态良好的PK15细胞,是生产优质疫苗的前提。随着疫苗生产工艺的不断更新迭代,细胞培养的方式也不断增加,目前常见的培养方式有方瓶、转瓶、生物反应器等。为了给生产制定更加优化的培养策略,本文探讨了PK15细胞在方瓶、转瓶、生物反应器三种不同培养条件下的生长情况。通过分析每次细胞培养前的分种密度、培养72 h细胞密度、细胞增长倍数并观察24 h、48 h、72 h的细胞状态,确定细胞的生长情况。结果表明,就对于细胞生长繁殖而言,无论哪种培养方法,该细胞均能很好生长。培养72 h,就细胞增长倍数而言,各培养系统有所区别,表现为方瓶倍增为10~30倍,转瓶为5~10倍,生物反应器为8~12倍。

PK15 cells are widely cultured in the study and production of mutiple veterinary vaccines. Culturing PK15 cells in good condition is the premise of producing high quality vaccine. With the continuous update of vaccine production process, methods of cell culture are increasing, such as square flask, rotating flask, bioreactor and so on. Here, in order to optimize culture methods for production, the growth of PK15 cells in square flask, rotating flask and bioreactor were studied. The growth of cells was determined by analyzing density of cells before culturing, density and growth multiple of cells incubating for 72 h and cell state of cells incubating for 24 h, 48 h and 72 h. The results showed that PK15 cell grow well in three different culture methods. In terms of cell growth multiple, it is different from three culture systems when PK15 cells were incubating for 72 h, which mainly manifested as 10 ~30 times in square flask, 5~10 times in rotating flask and 8~12 times in bioreactor.