MicroRNA-29c靶向CDC42调控MCF-7细胞的增殖与侵袭

MicroRNA-29c regulate the proliferation and invasion of MCF-7 cells in breast cancer by Targeting CDC42

目的:研究MicroRNA-29c(miR-29c)在乳腺癌中的表达及对乳腺癌MCF-7细胞增殖与侵袭的影响。方法:收集20例乳腺癌患者肿瘤组织和癌旁组织,利用qPCR检测miR-29c的表达情况;构建miR-29c过表达载体,转染MCF-7细胞,设立miR-29c组,NC组(转染空病毒)和control组,CCK8检测细胞增殖,Transwell检测细胞侵袭能力,同时利用双荧光素酶报告基因验证miR-29c靶基因。结果:乳腺癌组织中的miR-29c相对表达量明显低于癌旁正常组织,差异有统计学意义(P<0.05),MCF-7细胞过表达miR-29c后,细胞增殖能力显著低于NC组和control组,细胞侵袭能力显著低于NC组和control组(P<0.05),双荧光素酶报告系统显示miR-29c能够抑制靶基因CDC42载体荧光素酶活性。结论:miR-29c通过靶向CDC42蛋白抑制乳腺癌MCF-7细胞的增殖与侵袭,为乳腺癌的治疗提供候选分子靶点和理论依据。

Objective:To investigate the expression of MicroRNA-29c(miR-29c)in breast cancer and its effect on the proliferation and invasion of MCF-7 cells.Methods:Tumor tissue and paracancer tissue from 20 patients diagnosed with breast cancer were collected and the expression of miR-29c was detected by real-time PCR.miR-29c Over-expression Vector and empty Vector for control were constructed and transfected into MCF-7 cells.Then,CCK8 was used to detect cell proliferation,Transwell was performed to detect cell invasion and dual-luciferase reporter gene experiment was used to verify the reliability of CDC42 as the target gene of microRNA-29c.Results:The relative expression of miR-29c in breast cancer tissues was significantly lower than that in normal paracancer tissues(P<0.05).In addition,after the overexpression of miR-29c in MCF-7 cells,the proliferation ability of MCF-7 cells was significantly lower than that of control group,and the invasive ability of MCF-7 cells was weakened significantly(P<0.05).Moreover,the results of dual-luciferase reporter gene experiment showed that miR-29c inhibited the luciferase activity of CDC42.Conclusions:miR-29c inhibits the proliferation and invasion of MCF-7 cells by targeting CDC42 gene,providing a new molecular target and theoretical basis for the treatment of laryngeal squamous cell carcinoma.