敲减CDC7对膀胱癌T24细胞增殖、迁移、侵袭能力的影响

Effect of knockout CDC7 on proliferation,migration and invasion of bladder cancer T24 cells

目的:探讨敲减CDC7对人膀胱癌T24细胞增殖、迁移、侵袭能力的影响。方法:利用qRT-PCR方法检测正常膀胱组织及膀胱癌组织中CDC7的表达量,用siCDC7转染人膀胱癌T24细胞作为实验组,siNC转染人膀胱癌T24细胞作为对照组,转染24 h后,用CCK8实验、划痕实验、Transwell实验分别检测敲减CDC7对T24细胞增殖、侵袭、迁移能力的影响。结果:qRT-PCR结果显示与正常膀胱组织相比,CDC7在膀胱癌组织中的表达显著增高;CCK8结果显示实验组每个时间段的细胞生长数目较对照组明显减少;划痕实验的结果表明实验组T24细胞的划痕愈合面积较对照组减少;Transwell结果表明实验组穿过微孔膜的细胞数目较对照组明显减少。结论:敲减CDC7能够显著抑制膀胱癌T24细胞的增殖、迁移、侵袭能力。

Objective:To detect the effect of knockout CDC7 on proliferation,migration and invasion of human bladder cancer T24 cells.Methods:The expression of CDC7 in normal bladder tissue and bladder cancer tissue was detected by qRT-PCR.Human bladder cancer T24 cells were transfected with siCDC7 as experimental group,and siNC was transfected into T24 cells as control group.After transfection for 24 hours,CCK8 assay,scratch test and Transwell assay were used to detect the proliferation,invasion and migration of T24 cells by the knockdown of CDC7.Results:The results of qRT-PCR showed that the expression of CDC7 in bladder cancer tissues was significantly higher than that in normal bladder tissues.CCK8 showed that the number of cells in each time period of the experimental group was significantly reduced compared with the control group.The scratch healing area of T24 cells in the experimental group was lower than that in the control group,and the results of Transwell showed that the number of cells passing through the microporous membrane in the experimental group was significantly lower than that in the control group.Conclusions:Knockout CDC7 could significantly inhibit the proliferation,migration and invasion of bladder cancer T24 cells.