摘要
为提高脂肪酶催化合成酯类化合物的效率,优化催化条件,通过物理吸附将脂肪酶Pseudomonas cepacia lipase固定在脱脂棉上,与柱形瓶或滴定管一起形成简易的生物反应器,并用于催化乙酸乙烯酯和正戊醇反应,合成乙酸正戊酯。研究了温度、底物配比、溶剂用量等因素对转酯反应的影响,以及生物反应器的稳定性、构型和使用方法。研究发现,在静置的瓶型反应器中,37和25℃条件下,催化反应24 h,反应转化率可分别达到99%和97%。在管式反应器中,室温静置反应5 h,转化率可达77%。可见,制备的固定化酶反应器可在绿色和低碳条件下有效催化合成乙酸正戊酯。
In order to improve the efficiency of lipase-catalyzed synthesis of esters and optimize the catalytic conditions,Pseudomonas cepacia lipase was immobilized on absorbent cotton by physical adsorption to form a simple bioreactor with column bottle or titrator tube,and used to catalyze the reaction of vinyl acetate and n-amyl alcohol to synthesize n-amyl acetate.The effects of temperature,substrate ratio and solvent dosage on transesterification were studied,and also the stability,configuration and usage method of bioreactor were explored.It was found that the conversion of n-amyl alcohol to n-amyl acetate could achieve at 99% and 97% in an unshaken column bottle bioreactor at room temperature 37℃ and 25℃ after 24 hours,respectively.And the conversion also reached at 77% in a static tube bioreactor at room temperature after 5 h.It can be seen that the prepared enzymatic bioreactor can effectively catalyze the synthesis of n-amyl acetate under green and low carbon conditions.
作者
任德忠
丛方地
王晓红
张蒙
王鑫鑫
张树林
罗巍
REN De-zhong;CONG Fang-di;WANG Xiao-hong;ZHANG Meng;WANG Xin-xin;ZHANG Shu-lin;LUO Wei(College of Basic Sciences,Tianjin Agricultural University,Tianjin 300384,China;Tianjin Key Lab of Aqua-Ecology and Aquaculture,Tianjin 300384,China)
出处
《天津农学院学报》
CAS
2020年第1期65-69,共5页
Journal of Tianjin Agricultural University
基金
国家级大学生创新创业训练计划项目(201710061012,201810061072)
天津市水产生态及养殖重点实验室开放基金项目(TJAE201802)
天津自然科学基金重点项目(18JCZDJC97800)。
关键词
乙酸正戊酯
假单胞菌脂肪酶
脱脂棉
静置
转化率
n-amyl acetate
Pseudomonas cepacia lipase
absorbent cotton
static state
conversion