氧化壳聚糖改性抗菌蚕丝织物的制备及其性能

Preparation and properties of antibacterial silk fabric modified with oxidized chitosan

为获得持久抗菌的功能丝绸材料,采用硝酸、磷酸、亚硝酸钠体系将原料壳聚糖的C6位伯羟基选择性氧化为羧基制得水溶性氧化壳聚糖(OCS),使OCS的羧基与脱胶蚕丝织物(SFF)的氨基反应,得到氧化壳聚糖改性蚕丝织物(OCSMSF)。借助核磁共振仪、扫描电子显微镜、红外光谱仪、X射线衍射仪等手段表征了OCS与OCSMSF的化学结构,测试了OCSMSF的力学、吸湿、抗菌等性能。结果表明:酰胺反应已将氧化壳聚糖分子化学键合在SFF中,OCS改性蚕丝纤维的结晶度降低;在优化反应参数条件下,OCS接枝蚕丝织物的质量增加率达到9. 17%,OCSMSF的强力略有减小,而吸湿性提高42. 92%,OCSMSF对测试细菌的抑菌率大于94%,且抗菌耐洗涤性好,同时OCSMSF对仙人掌黄酮提取物有良好的缓释效果。

In order to acquire durable antibacterial silk materials,the hydroxyl of C6 site in original chitosan( CS) was selectively oxidized to the carboxyl by HNO3/H3 PO4-NaNO2 mediate system and obtained the water-soluble oxidized chitosan( OCS),and then the chemical bond was formed through the amide reaction between carboxyl groups in oxidized chitosan and amino groups of silk fibroin fabric( SFF) to get antimicrobial silk fibroin materials( OCSMSF). Nuclear magnetic resonance,Scanning electron microscopy,Infrared spectroscopy and X-ray diffraction were used to test the chemical structure of OCS and OCSMSF,moreover,the mechanics,hygroscopicity and antibacterial activity of OCSMSF materials were also measured. The results suggest that the carboxylic chitosan molecules are crosslinked into SFF by the amide reaction,and the degree of crystallinity for SFF materials grafted with OCS decrease. The oxidized chitosan weight gain of 9. 17% for the modified silk fabric is achieved under the condition of optimized reaction parameters,the tensile strength of grafted silk fabrics reduces slightly,whereas the moisture adsorption of the modified SFF increases 42. 92%. Furthermore,the antimicrobial ratio of the grafted SFF against the tested bacteria exceedes 94%,as well as still high antibacterial activity after washing;meanwhile,a good efficiency of sustained release cactus flavonoid extract using the OCSMSF is performed. The OCS grafted silk materials have potential application prospect in antibacterial materials or carrier materials of release drug.