血浆长链非编码RNA AFAP1-AS1和SOX2OT的表达对非小细胞肺癌的诊断价值

Diagnostic value of plasma lncRNA AFAP1-AS1 and lncRNA SOX2OT in non-small cell lung cancer

目的:探讨血浆长链非编码RNA(long non-coding RNA,lncRNA)肌动蛋白纤维相关蛋白1-反义RNA1(actin filament-associated protein 1-antisense RNA1,AFAP1-AS1)和Y染色体性别决定基因簇2重叠转录本(sex-determining region of Y chromosome-box2 overlapping transcript,SOX2OT)表达水平对于非小细胞肺癌(non-small cell lung cancer,NSCLC)的临床诊断价值。方法:留取48例NSCLC患者(NSCLC组)和48例良性肺病患者(对照组)血液标本,采用实时定量PCR检测lncRNA AFAP1-AS1和lncRNA SOX2OT相对表达水平,评价二者诊断NSCLC的可行性及效能,同时检测血清癌胚抗原水平。结果:NSCLC患者血浆lncRNA AFAP1-AS1和lncRNA SOX2OT表达水平显著高于对照组(t=6.236,5.680,P均<0.01),lncRNA AFAP1-AS1和lncRNA SOX2OT鉴别诊断NSCLC与良性肺病的受试者工作特征曲线下面积(AUC)分别为0.875(95%CI:0.804~0.947,P<0.05)和0.787(95%CI:0.691~0.883,P<0.05)。二者联合血清癌胚抗原可提高诊断的AUC。结论:NSCLC患者血浆中lncRNA AFAP1-AS1和lncRNA SOX2OT表达明显上调,二者联合血清癌胚抗原对NSCLC的诊断效能优于单独应用。

Objective:To assess the diagnostic value of plasma long non-coding RNA(lncRNA)actin filament-associated protein 1-antisense RNA1(AFAP1-AS1)and lncRNA sex-determining region of Y chromosome-box2 overlapping transcript(SOX2OT)as biomarkers in non-small cell lung cancer(NSCLC).Methods:Blood samples of 48 non-small cell lung cancer(NSCLC)patients(NSCLC group)and 48 benign lung disease patients(control group)were collected.The relative expression levels of lncRNA AFAP1-AS1 and lncRNA SOX2OT were detected by real-time quantitative PCR to evaluate the diagnostic performance of the two lncRNAs as biomarkers for NSCLC.The level of serum carcinoembryonic antigen was also detected.Results:Compared with the control group,the levels of plasma lncRNA AFAP1-AS1 and lncRNA SOX2OT were significantly increased in NSCLC patients(t=6.236,5.680,both P<0.01).The area under the receicer operating characteristic curve(AUC)of the two plasma lncRNAs which were used to distinguish NSCLC patients from control group are 0.875(95%CI:0.804~0.947,P<0.05)for lncRNA AFAP1-AS1 and 0.787(95%CI:0.691~0.883,P<0.05)for lncRNA SOX2OT.Combined detection of these two lncRNAs and serum carcinoembryonic antigen could improve the diagnostic AUC.Conclusion:NSCLC patients showed higher levels of plasma lncRNA AFAP1-AS1 and lncRNA SOX2OT,combination of which and serum carcinoembryonic antigen could improve diagnosis efficiency for NSCLC.