不同CO2浓度对普通小球藻生化组成及光合生理的影响

Effects of Different CO2 Concentrations on the Biochemical Composition and Photosynthetic Physiology of Chlorella Vulgaris

探讨了普通小球藻对于CO2浓度升高的生理响应。在5个CO2浓度下(400,700,1000,1500和2000μatm)(1 atm=1.01×10^5 Pa)静止培养小球藻20代后,对其光合生理和生化组成进行分析。其中,升高CO2浓度增加了普通小球藻的细胞密度,且1000μatm是普通小球藻生长的最适浓度。实际光合效率与细胞密度呈现出相似的趋势。色素含量在1000、1500及2000μatm显著高于其他CO2浓度。CO2浓度升高会降低普通小球藻可溶性糖的含量,增加其蛋白质含量。除此之外,随着CO2浓度的升高,总酚物质的含量逐步后趋于平缓的趋势。油脂含量在1500和2000μatm下显著高于其他CO2浓度下小球藻的油脂含量。当CO2浓度为700,1000,1500和2000μatm时,碳氮比与400μatm时相比均发生了极显著的变化。不同CO2浓度下的不同生物组成的变化趋势有助于对未来环境下浮游植物对环境的响应做进一步研究。

The physiological responses of Chlorella vulgaris to increased CO2 concentrations(400,700,1000,1500 and 2000μatm)(1 atm=1.01×10^5 Pa)were investigated in this study.Photosynthetic physiology and biochemical composition of Chlorella vulgaris were measured after 20 generations of culture under elevated CO2 concentrations.The cell density increased significantly under elevated CO2 concentrations especially under 1000μatm.Actual photosynthesis efficiency and cell density showed similar trend which exhibited the most marked increase under 1000μatm.The difference between the pigments at the higher CO2 concentrations(1000,1500 and 2000μatm)was little but much higher than other CO2 concentrations.Elevated CO2 has a significant inhibition on soluble carbohydrates.Within the limit of CO2 concentration,the increase of CO2 concentration will promote the protein content of Chlorella vulgaris.The lipid content and n(C)/n(N)under high CO2 concentrations changed significantly compared to 400μatm.The trend of different biological compositions at different CO2 concentrations is benefit to further study of the response of phytoplankton to the environment in the future.