玉木耳转录组测序及褐变相关基因的挖掘

Transcriptome Sequencing of Auricularia cornea and Investigating of Genes Involved in Browning

为了从分子水平研究玉木耳褐变的机理,对褐变和未褐变的玉木耳子实体进行了转录组测序分析,共筛选到5915个差异基因,其中有924个Unigene上调表达,有4991个Unigene下调表达。通过基因本体(Gene Ontology,GO)富集分析发现,差异基因显著富集于细胞及代谢过程、单一有机体、细胞和细胞组分、细胞器和大分子复合体、结合作用和催化活性。KEGG代谢通路富集分析表明,富集程度比较显著的通路包括翻译通路、信号转导途径、内分泌系统、折叠排序及退化、运输和分解代谢通路。挑选与组织褐变相关的差异基因进行qRT-PCR表达模式验证,获得的结果与表达谱分析结果一致。

In order to study the mechanism of the browning of Jade fungus from the molecular level,we performed transcriptome sequencing analysis on browning and no browning.After preliminary analysis of the sequencing data,a total of 5915 differential genes were screened,of which 924 Unigenes were up-regulated and 4991 Unigenes were down-regulated.Through gene ontology(GO)enrichment analysis,it was found that differential genes were significantly enriched in cells and metabolic processes,single organisms,cell and cell components,organelles and macromolecular complexes,binding and catalytic activity.Enrichment analysis of the differentially expressed gene KEGG metabolic pathway showed that the pathways with significant enrichment including translation pathway,signal transduction pathway,endocrine system,folding sorting and degradation,transport and catabolic pathway.Differential genes associated with tissue browning were selected to verify by qRT-PCR expression pattern,and the obtained results were consistent with the results of expression profiling.