脂肪干细胞外泌体可促进大鼠海绵体神经再生

Exosomes from adipose-derived stem cells can promote cavernous nerve regeneration in rats

背景:海绵体神经是调控阴茎勃起的重要神经,在盆腔手术或外伤中容易受到损伤,导致阴茎勃起功能障碍。干细胞来源外泌体对于受损海绵体神经再生的直接影响目前尚无研究报道。目的:观察脂肪干细胞外泌体对海绵体神经再生的影响。方法:体外构建大鼠盆大神经节三维培养模型,培养24 h后实验组换成含有青霉素-链霉素和脂肪干细胞来源外泌体的RPMI-1640培养基,外泌体浓度为1015 L-1,对照组仍使用含有青霉素-链霉素的RPMI-1640培养基。干预开始后第2,5,9天观察两组海绵体神经断端再生情况,并测量轴突生长长度,用以评价海绵体神经再生能力。结果与结论:①从大鼠肾周脂肪组织获得的原代脂肪干细胞贴壁生长,形态稳定,能够诱导分化为脂肪、骨和软骨;②提取自第3代脂肪干细胞的外泌体呈大小均一的囊泡结构,直径集中在85 nm附近;③三维培养体系中的海绵体神经自身具有一定的再生能力,外泌体干预后2,5,9 d实验组海绵体神经再生情况均优于对照组,轴突再生长度明显大于对照组;④结果表明,脂肪干细胞来源外泌体直接作用于海绵体神经,对其再生具有显著的促进作用。

BACKGROUND:Cavernous nerve is one of the main nerves regulating penile erection,which is easy to be damaged in pelvis surgery and due to trauma and induce erectile dysfunction.The direct effect of stem cell-derived exosome on the regeneration of injured cavernous nerve has not been reported yet.OBJECTIVE:To observe the effect of the exosomes from adipose-derived stem cells on the regeneration of cavernous nerve.METHODS:Three-dimensional culture model of rat nerve tissue in vitro was constructed.After 24 hours of incubation,the experimental group was incubated in the RPMI-1640 medium containing penicillin-streptomycin and exosomes(1015/L)from adipose-derived stem cells.The control group was incubated in the RPMI-1640 medium.The length of axon regeneration at the incisal edge of the cavernous nerve was measured to evaluate the regeneration ability of the cavernous nerve at 2,5 and 9 days after intervention.RESULTS AND CONCLUSION:Adipose-derived stem cells extracted from rat perirenal adipose tissue adhered with stable morphology and could be induced to differentiate into adipose,bone and cartilage.The exosomes extracted from the passage 3 adipose-derived stem cells showed similar vesicle structure with a diameter near 85 nm.In the three-dimensional culture system,the regeneration ability of the cavernous nerve itself was certain.The axon regeneration length in the experimental group was significantly longer than that in the control group at 2,5 and 9 days after intervention.The exosomes from adipose-derived stem cells directly act on the cavernous nerve and have a significant promoting effect on nerve regeneration.