复方甘草酸苷对肝癌合并肝硬化的作用研究

Effect of stronger Neo-Mioniphagen C in hepatocarcinoma associated with cirrhosis

目的:分析复方甘草酸苷(SNMC)对肝癌合并肝硬化患者肝功能的影响,并探讨该药物对肝癌细胞系Hep-G2、MHCC-97H增殖能力的影响。方法:160例肝癌合并肝硬化患者作为研究对象,随机分为观察组和对照组,观察并比较两组患者治疗前后各项肝功能指标变化、以不同浓度SNMC(0、25、100、200、800μg/mL)分别处理肝癌细胞系Hep-G2及MHCC-97H,应用CCK-8细胞增殖实验、平板集落形成实验探究其对肝癌细胞增殖能力的影响。结果:观察组治疗后AST、ALT、TBIL均明显低于对照组,ALB明显高于对照组,差异均有统计学意义(P<0.05);随SNMC药物浓度的增加,肝癌细胞的增殖能力逐渐降低。100、200、800μg/mL的SNMC可显著降低肝癌细胞的增殖,差异均有统计学意义(P<0.05)。结论:SNMC对肝癌合并肝硬化患者具有保肝作用,可促进肝功能的恢复、延缓肝功能的恶化、提高患者的生存质量,其机制可能通过抑制肝癌细胞的增殖而实现。

Objectives:To analyze the effect of Stronger Neo-Mioniphagen C(SNMC)on liver function in patients with hepatocarcinoma and cirrhosis,and to explore the effect of the drug on the proliferation ability of hepatocellular carcinoma cell lines HepG2,MHCC-97H.Methods:160 patients with liver cancer and liver cirrhosis were taken as the research objects,and they were randomly divided into observation group and control group.Observing and comparing various liver function indexes in the two groups of patients.Meanwhile,different concentrations of SNMC(0,25,100,200,800μg/mL)were used to treat the hepatocellular carcinoma cell lines Hep-G2 and MHCC-97H,respectively.CCK-8 and plate colony formation assay were used to explore the effect of SNMC on the proliferation of hepatoma cells.Results:After surgery,AST,ALT,and TBIL in the observation group were significantly lower than those in the control group,and ALB was markedly higher than the control group(P<0.05).with the increase of the concentration of SNMC,the proliferation of hepatocellular carcinoma cells gradually increased.SNMC at 100,200,and 800μg/mL can obviously reduce the proliferation of hepatocellular carcinoma cells(P<0.05).Conclusion:SNMC has a hepatoprotective effect on patients with liver cancer and cirrhosis,which can promote the recovery of liver function,delay the deterioration of liver function,and improve the quality of patients lives.The mechanism may be achieved by inhibiting the proliferation of liver cancer cells.