miR-34a、CD133在胰腺癌中的表达及临床意义

Expression and clinical significance of miR-34a and CD133 in pancreatic cancer

目的:检测胰腺癌肿瘤组织中微小RNA-34a与CD133表达情况,并探究其临床意义。方法:选取2012年12月至2014年2月本院收治的胰腺癌患者74例,取胰腺癌肿瘤组织及癌旁组织。采用实时定量PCR(qRT-PCR)法检测胰腺癌肿瘤组织及癌旁组织中miR-34a表达水平,采用免疫组化法检测CD133表达水平。结果:胰腺癌肿瘤组织miR-34a表达水平低于癌旁组织(P<0.05),CD133 mRNA表达水平较癌旁组织显著升高(P<0.05);CD133在胰腺癌肿瘤组织中阳性表达率显著高于癌旁组织(P<0.05)。miR-34a、CD133表达与胰腺癌肿瘤分化程度、肿瘤大小、淋巴结转移有关(P<0.05);miR-34a、CD133表达与胰腺癌患者年龄、性别无显著相关性(P>0.05)。miR-34a低表达胰腺癌患者3年总生存率显著低于高表达患者(P<0.05),CD133高表达胰腺患者3年总生存率显著低于CD133低表达者(P<0.05)。miR-34a、CD133表达、肿瘤大小和肿瘤分化程度是影响胰腺癌患者预后的独立危险因素。胰腺癌肿瘤组织miR-34a与CD133 mRNA相对表达水平显著负相关(P<0.05)。结论:miR-34a在胰腺癌组织中低表达,CD133高表达,与患者肿瘤分化程度、肿瘤大小、临床分期有关,可能作为胰腺癌病情监测及术后预后的生物学指标。

Objective:To investigate the expressions and clinical significances of miR-34 a and CD133 in pancreatic cancer.Methods:74 patients with pancreatic cancer in our hospital from December 2012 to February 2014 were selected.The tumor tissues and adjacent tissues of pancreatic cancer were obtained.Real-time quantitative PCR(qRT-PCR)was used to detect the expression of miR-34 a in pancreatic cancer tissues and adjacent tissues.The expression level of CD133 was detected by immunohistochemistry.Results:The expression level of miR-34 a in pancreatic cancer tissues was lower than that in adjacent tissues(P<0.05).The expression level of CD133 mRNA inpancreatic cancer tissues was higher than that in adjacent tissues(P<0.05).The positive expression rate of CD133 in pancreatic cancer tissues was significantly higher than that in adjacent tissues(P<0.05).miR-34 a and CD133 expressions were correlated with tumor differentiation,tumor size and lymph node metastasis(P<0.05).miR-34 a and CD133 expressions were not correlated with age and gender of pancreatic cancer patients(P>0.05).The 3-year overall survival rate of pancreatic cancer patients with miR-34 a low expression was significantly lower than that of patients with high expression(P<0.05).The 3-year overall survival rate of pancreatic cancer patients with CD133 high expression was significantly lower than that of those with low expression(P<0.05).The expressions of miR-34 a,CD133,tumor enlargment and the degree of tumor differentiation were independent risk factors for the prognosis of pancreatic cancer.The expression level of CD133 mRNA was negatively correlated with miR-34 a.Conclusion:miR-34 a is low expressed in pancreatic cancer tissues,and CD133 is highly expressed.They are related to tumor differentiation,tumor enlargement and clinical stage,and may be used as biomarkers for monitoring and prognosis of pancreatic cancer.