miR-155和TGFβR2在结肠癌中的表达及临床意义

Expression and clinical significance of miR-155 and TGFβR2 in colon cancer

目的:探讨结肠癌组织中微小RNA-155(miR-155)与转化生长因子β受体Ⅱ(TGFβR2)的表达及其临床意义。方法:选取接受手术治疗的97例结肠癌患者为研究对象,将癌组织及其癌旁组织标本分别作为结肠癌组、正常组。采用qRT-PCR法检测两组miR-155与TGFβR2 mRNA相对表达量,免疫组化法检测组织中TGFβR2蛋白表达。根据miR-155检测结果的平均值分组,高于平均值为高表达组,低于平均值为低表达组,根据免疫组化检测结果将TGFβR2分为阳性表达组与阴性表达组,观察其与患者临床病理参数的关系。采用Pearson法分析miR-155与TGFβR2的相关性;Kaplan-Meier对结肠癌患者3年的生存情况进行分析。结果:与正常组相比,结肠癌组miR-155表达水平显著升高(P<0.05),而TGFβR2 mRNA及蛋白表达显著降低(P<0.05);miR-155、TGFβR2表达均与淋巴结转移、临床分期、组织分化程度、浸润深度显著相关(P<0.05);miR-155与TGFβR2呈显著负相关(P<0.05);miR-155低表达组PFS、OS均显著高于高表达组(P<0.05),TGFβR2阳性表达组PFS、OS均显著高于阴性表达组(P<0.05)。结论:结肠癌组织中高表达的miR-155与低表达的TGFβR2均可作为预测癌症进展情况的有效指标,二者呈负相关且异常表达均与患者预后不良密切相关。

Objective:To investigate the relationship between microRNA-155(miR-155)and transforming growth factor beta receptor II(TGFβR2)in colon cancer and its clinical significance.Methods:The 97 patients with colon cancer who underwent surgery were selected as the study subjects.The cancer tissues and their adjacent tissues were used as the colon cancer group and the normal group.The relative expression levels of miR-155 and TGFβR2 mRNA were detected by qRT-PCR.TGFβR2 protein expression was detected in tissues by immunohistochemistry.The average value of the results based on miR-155 will be higher than the average for the high expression group.According to the results of immunohistochemistry,TGFβR2 was divided into positive expression group and negative expression group.And its relationship with clinical pathological parameters of patients was observed.The correlation between miR-155 and TGFβR2 was analyzed by Pearson method.Kaplan-Meier analyzed the 3-year survival of colon cancer patients.Results:Compared with the normal group,the expression level of miR-155 in colon cancer group was significantly increased(P<0.05),while the expression of TGFβR2 mRNA and protein was significantly decreased(P<0.05).The expressions of miR-155 and TGFβR2 were significantly correlated with lymph node metastasis,clinical stage,degree of tissue differentiation and depth of invasion(P<0.05).There was a significant negative correlation between miR-155 and TGFβR2(P<0.05).PFS and OS in miR-155 low expression group were significantly higher than those in high expression group(P<0.05).PFS and OS in TGFβR2 positive expression group were significantly higher than those in negative expression group(P<0.05).Conclusion:The high expression of miR-155 and low expression of TGFβR2 in colon cancer can be used as effective indexes to predict the progression of cancer.The negative correlation and abnormal expression of the two are closely related to the poor prognosis of the patients.