葛根红藤解毒汤对溃疡性结肠炎大鼠结肠组织中TRL4及血清白细胞介素-10的影响

Effects of Gegen Hongteng Jiedu Decoction on TRL4 and IL-10 in rats with ulcerative colitis

目的:观察葛根红藤解毒汤对溃疡性结肠炎(UC)大鼠结肠组织中TLR4蛋白的表达及血清白细胞介素-10(IL-10)含量的影响,并探讨其作用机制。方法:SPF级Wistar雄性大鼠60只,随机选取10只作为空白组,剩余大鼠采用2,4,6-三硝基苯磺酸(TNBS)/乙醇法制备UC大鼠模型。造模成功后,随机分为模型组、柳氮磺吡啶组、葛根红藤解毒汤组,每组各15只,予灌胃治疗14d。评估大鼠的一般情况、疾病活动指数(DAI),观察TLR4及IL-10的变化。结果:与空白组比较,模型组大鼠DAI评分、TLR4蛋白表达增高(P<0.05),血清IL-10含量降低(P<0.05)。与模型组比较,柳氮磺吡啶组、葛根红藤解毒汤组大鼠DAI评分、TLR4蛋白表达均降低(P<0.05),血清IL-10含量增高(P<0.05)。葛根红藤解毒汤组与柳氮磺吡啶组比较,两组各项指标差异均无统计学意义。结论:葛根红藤解毒汤治疗UC大鼠的机制可能是降低结肠组织中TLR4蛋白的表达,并提高血清IL-10的含量。

Objective:To observe the effects of Gegen Hongteng Jiedu Decoction on toll-like receptor 4(TLR4)and serum interleukin-10(IL-10)in rats with ulcerative colitis(UC),and to analyze the possible mechanism of it.Methods:A total of 60 SPF Wistar male rats were randomly divided into blank group with 10 rats and experimental group.In experimental group,UC animal model was replicated with 2,4,6-trinitrobenzenesulfonic acid(TNBs)/ethanol method,and they were randomly divided into model group,SASP group and Gegen Hongteng Jiedu Decoction group with 15 rats in each one.Give them corresponding durg,and gavage for 14 days.Judging the rats’general conditions,and scored according to disease activity index(DAI),and to observe the changes of TLR4 and IL-10.Results:Compared with blank group,other groups’DAI score and the expression level of TLR4 were increased(P<0.05),while the IL-10 content of serum was decreased(P<0.05).Compared with model group,DAI score and the expression level of TLR4 were decreased(P<0.05)in SASP group and Gegen Hongteng Jiedu Decoction group,while the IL-10 content of serum was increased(P<0.05).There was no statistical significance of Gegen Hongteng Jiedu Decoction group and SASP group.Conclusion:The possible mechanism of Gegen Hongteng Jiedu Decoction may to reduce the expression level of TLR4 and improve the IL-10 content of serum.