摘要
环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种新型的由环介导的等温核酸扩增分子技术,不仅特异性强、操作简便、成本低,还能快速、高效地检测病原物,为植物病害的防控提供更精准的防治适期,从而可以减少农药的滥用。本文主要针对LAMP技术的原理、发展、优缺点、在真菌、细菌、病毒等多种植物病原物检测及在抗药性检测中的应用进行总结,并结合国内外研究进展对其应用前景进行了分析。
Loop-mediated isothermal amplification(LAMP)technology is a new type of loop-mediated isothermal nucleic acid amplification molecular technology,which has significant advantages such as strong specificity,easy operation and low cost,and was used to fast,high efficiently dectect patheogens.LAMP technology provides more accurate control period for the effective prevention and control of plant diseases,so as to reduce the abuse of pesticide.This paper mainly summarizes the principle,development,advantages and disadvantages of LAMP technology,and its application in the detection of various plant pathogens such as fungi,bacteria,and viruses,as well as the detection for pesticide resistance,and analyzes its application prospects in accordance with domestic and foreign research progress.
作者
应淑敏
郭俭
王教瑜
李玲
张传清
YING Shumin;GUO Jian;WANG Jiaoyu;LI Ling;ZHANG Chuanqing(College of Agricultural and Food Sciences,Zhejiang Agriculture and Forestry University,Lin’an 311300,Zhejiang Province,China;Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2020年第2期234-244,共11页
Journal of Plant Protection
基金
浙江省重点研发项目(2018C02G2011099)
国家自然科学基金(31701723)
浙江农林大学学生科研训练项目(KX20180020)。
关键词
LAMP技术
检测
植物病原物
loop-mediated isothermal amplification(LAMP)
detection
plant pathogens