环介导等温扩增技术在植物病原物检测中的应用

Application of LAMP in the detection of plant pathogens

环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种新型的由环介导的等温核酸扩增分子技术,不仅特异性强、操作简便、成本低,还能快速、高效地检测病原物,为植物病害的防控提供更精准的防治适期,从而可以减少农药的滥用。本文主要针对LAMP技术的原理、发展、优缺点、在真菌、细菌、病毒等多种植物病原物检测及在抗药性检测中的应用进行总结,并结合国内外研究进展对其应用前景进行了分析。

Loop-mediated isothermal amplification(LAMP)technology is a new type of loop-mediated isothermal nucleic acid amplification molecular technology,which has significant advantages such as strong specificity,easy operation and low cost,and was used to fast,high efficiently dectect patheogens.LAMP technology provides more accurate control period for the effective prevention and control of plant diseases,so as to reduce the abuse of pesticide.This paper mainly summarizes the principle,development,advantages and disadvantages of LAMP technology,and its application in the detection of various plant pathogens such as fungi,bacteria,and viruses,as well as the detection for pesticide resistance,and analyzes its application prospects in accordance with domestic and foreign research progress.