淫羊藿苷对家兔腰椎间盘退变的影响

Effect of icariin on lumbar disc degeneration in rabbits

目的:观察淫羊藿苷对退变家兔腰椎间盘中基质金属蛋白酶13(MMP-13)、基质金属蛋白酶组织抑制因子-1(TIMP-1)以及细胞凋亡因子Bax、Bcl-2蛋白表达的影响。方法:30只新西兰大白兔随机分为空白组、模型组和实验组,每组各10只。用髓核穿刺抽吸法复制腰椎间盘退变模型。术后4周,实验组用淫羊藿苷(3 mg/kg·d)灌胃,空白组和模型组用等量0.9%氯化钠注射液灌胃,连续4周。分别于术后4周和术后8周采用免疫组化法检测椎间盘中MMP-13、TIMP-1水平,Western blot检测椎间盘中Bax、Bcl-2水平。结果:MMP-13、TIMP-1、Bax、Bcl-2表达水平实验组术后4周及模型组术后4、8周与同时期空白组相比,实验组术后8周Bax、Bcl-2表达水平与模型组同时期比较,模型组、实验组术后8周Bax、Bcl-2表达水平与术后4周组内比较,差异均有统计学意义(P<0.05)。结论:淫羊藿苷可通过下调退变椎间盘中MMP-13、Bax的表达,上调TIMP-1、Bcl-2的表达,而起到防治腰椎间盘退变的作用。

Objective:To investigate the effect of icariin on the expression of matrix metalloproteinase-13(MMP-13),tissue inhibitor of metalloproteinase-1(TIMP-1),and the apoptotic factors Bax and Bcl-2 in in the degenerated lumbar disc in rabbits.Methods:A total of 30 New Zealand white rabbits were randomly divided into blank group,model group,and experimental group,with 10 rabbits in each group.Nucleus pulposus puncture and aspiration were performed to establish a model of lumbar disc degeneration.At 4 weeks after surgery,the rabbits in the experimental group were given icariin(3 mg/kg·d)by gavage,and those in the blank group and the model group were given an equal volume of 0.9%sodium chloride by gavage,both for 4 consecutive weeks.At 4 and 8 weeks after surgery,immunohistochemistry was used to measure the levels of MMP-13 and TIMP-1 in the intervertebral disc,and Western blot was used to measure the levels of Bax and Bcl-2 in the intervertebral disc.Results:There were significant differences in the levels of MMP-13,TIMP-1,Bax,and Bcl-2 between the experimental group and the blank group at 4 weeks after surgery(P<0.05),as well as between the model group and the blank group at 4 and 8 weeks after surgery(P<0.05).There were significant differences in the levels of Bax and Bcl-2 between the experimental group and the model group at 8 weeks after surgery(P<0.05).The model group and the experimental group had significant changes in the expression of Bax and Bcl-2 from 4 to 8 weeks after surgery(P<0.05).Conclusion:Icariin can be used to prevent and treat lumbar disc degeneration by downregulating the expression of MMP-13 and Bax and upregulating the expression of TIMP-1 and Bcl-2 in the degenerated intervertebral disc.