摘要
植物重要性状相关的数量性状基因座(QTL)和基因定位一直是结构基因组学的研究重点,也是遗传机制解析和分子育种的重要基础。木本植物具有重要的经济、生态价值,但用于其研究的传统基因定位方法存在耗时长、工作量大、通量低、成本高且效果不佳等问题。近年来,由于测序技术发展日新月异,基于二代测序技术的集群分离分析法(NGS-based BSA)开始应用于木本植物中。与传统的集群分离分析法(BSA)技术相比,NGS-based BSA周期短、效率高,能对质量或数量性状进行精确定位,在木本植物遗传学和组学研究方面具有广阔的应用前景。文中简述了BSA的发展历程和研究策略,综述了其在木本植物研究中的进展,分析了NGS-based BSA的优势及存在的问题,展望了其在木本植物遗传学、组学和种质改良领域中的应用潜力。
Mapping of functional QTLs and genes relevant to plant traits is always the focus of structural genomics,which also lays a solid foundation for the genetic mechanism deciphering and molecular breeding.Woody plants have significant economic and ecological values.However,the common genetic mapping approaches adopted for woody plant analysis are time-consuming,laboring,low-fluxes,costly and inefficient.Recently,next-generation sequencing-based bulked segregant analysis(BSA)has been used in woody plant QTL and gene mapping with the rapid development of the sequencing technology.Compared with conventional BSA,it has the advantages like short time period,high efficiency and accurate positioning of quality or quantitative traits,which has great potential to be used in the genetics and genomics of woody plants.The paper summarizes the development and strategies of BSA,reviews its latest research progresses in woody plants,analyzes its advantages and extant problems,and prospects its potential to be applied in genetics,genomics and germplasm improvement in woody plants.
作者
梁晓涵
刘婷婷
张烨
程堂仁
王佳
张启翔
潘会堂
Liang Xiaohan;Liu Tingting;Zhang Ye;Cheng Tangren;Wang Jia;Zhang Qixiang;Pan Huitang(Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban andRural Ecological Environment,School of LandscapeArchitecture,Beijing Forestry University,Beijing 100083,China)
出处
《世界林业研究》
CSCD
北大核心
2020年第2期54-61,共8页
World Forestry Research
基金
北京市科技计划课题“基于组学的北京特色花灌木优良新品种培育及应用”(Z181100002418006)
国家自然科学基金“利用转录组测序技术挖掘调控紫薇株高性状的关键基因”(31470695)。
