7种挥发性化合物对柑橘木虱引诱效果的评价

Evaluation of attraction effect of seven volatile compounds on Diaphorina citri Kuwayama

柑橘黄龙病对全球的柑橘产业造成了严重的经济损失,其传播媒介主要为柑橘木虱。在前期研究中利用柑橘木虱对4种芸香科植物的取食偏好结合代谢组学分析筛选出一批可能对柑橘木虱具有引诱效果的挥发性化合物,本研究鉴定了邻氨基苯甲酸甲酯、癸酸、正辛醇、左旋香芹酮、2-环己烯酮、反式-1,2-环己二醇、吡嗪等7种挥发性化合物在0、0.1%、1%、10%4个浓度和10、20、30、40、50、60 min 6个持续时间下对柑橘木虱的引诱效果,并比较了其中5种脂溶性挥发物在各自最优浓度下的引诱效果。试验结果表明,7种挥发物对柑橘木虱都有引诱效果,但是引诱效果与挥发物浓度及持续时间有关。吡嗪、左旋香芹酮、正辛醇、2-环己烯酮、癸酸、邻氨基苯甲酸甲酯和反式-1,2-环己二醇的最佳引诱浓度分别为0.1%、1%、1%、1%、10%、10%、10%。六臂嗅觉仪试验结果显示5种脂溶性挥发物中1%左旋香芹酮的引诱效果高于10%癸酸、10%邻氨基苯甲酸甲酯、1%2-环己烯酮、1%正辛醇及对照(矿物油),但与其他4种挥发物没有显著差异。研究结果可为柑橘木虱引诱剂的开发提供参考,促进柑橘黄龙病的绿色防控。

Huanglongbing has caused great economic loss to the citrus industry globally, which is mainly spread by Diaphorina citri Kuwayama. Based on host plant preference and metabolic profiling, a number of volatile organic compounds(VOCs) that may attract D.citri have been identified from four Rutaceae host plants in our previous study. Here seven VOCs were selected and their attractive effect on D.citri was analyzed at four concentrations(0, 0.1%, 1%, 10%) and six duration(10, 20, 30, 40, 50, 60 min), namely methyl anthranilate, decanoic acid, n-octanol, trans-1,2-cyclohexanediol, pyrazine, l-carvone and 2-cyclohexen-1-one. Meanwhile, the responses of D.citri to five fat-soluble VOCs at their respective best attracting concentration were compared. The results revealed that these seven VOCs were all attractive to D.citri and the attracting effects varied depending on concentration and experiment duration. The best attracting concentrations of pyrazine, l-carvone, n-octanol, 2-cyclohexen-1-one, decanoic acid, methyl anthranilate and trans-1,2-cyclohexanediol were 0.1%, 1%, 1%, 1%, 10%, 10% and 10%, respectively. Six-armed olfactometer detection suggested that 1% l-carvone was more attractive to D.citri than 10% decanoic acid, 10% methyl anthranilate, 1% 2-cyclohexen-1-one, 1% n-octanol and the control(mineral oil), but there was no significant difference between l-carvone and the other 4 volatiles. Our result may provide alternatives for the development of environment-friendly D.citri attractant and promote the green prevention and control of citrus Huanglongbing.