我国云南、湖北和山东葡萄产区霜霉病菌对甲霜灵的抗性检测

Detection of resistance of Plasmopara viticola to metalaxyl in grapevine areas of Yunnan, Hubei and Shandong in China

为明确我国云南省宾川县、湖北省公安县和山东省烟台市葡萄产区霜霉病菌对甲霜灵的抗性发生态势,采用叶盘漂浮法测定了这3个产区共127株葡萄霜霉病菌对甲霜灵的抗性频率及抗性水平。结果显示,不同区域间病菌的抗性频率和抗性水平均存在差异。其中,湖北省公安县霜霉病菌的抗性频率和抗性水平均较高,抗性频率达92.0%,高抗菌株占76.0%,低抗菌株占16.0%,敏感菌株占8.0%;山东省烟台市霜霉病菌的抗性频率为74.0%,低抗菌株占64.0%,高抗菌株占10.0%,敏感菌株占26.0%;云南省宾川县霜霉病菌的抗性频率和抗性水平均较低,抗性频率为29.6%,敏感菌株占70.4%,低抗菌株占29.6%,无高抗菌株。

In order to clarify the development of fungicide resistance of Plasmopara viticola to metalaxyl in Binchuan county, Yunnan province, Gongan county, Hubei province, and Yantai city, Shandong province in China, 127 isolates of P.viticola collected from these regions were investigated for their resistance frequency and resistance level to the metalaxyl of P.viticola with leaf disk floating method. The results showed that resistance frequency and levels of P.viticola were different among isolates from different regions. The resistance frequency and levels of P.viticola isolates in Gongan county of Hubei province were both higher, with the frequency of 92.0%, among which, the high-resistance type isolates accounted for 76.0%, the low-resistance type accounted for 16.0%, and the sensitive type accounted for 8.0%. The resistance frequency of the P.viticola in Yantai city of Shandong province was 74.0%, the low-resistant isolates accounted for 64.0%, high-resistant isolates accounted for 10.0%, and sensitive isolates accounted for 26.0%. However, the resistance frequency and levels of isolates in Binchuan county of Yunnan province were both lower, and the resistance frequency was 29.6%, the sensitive isolates accounted for 70.4%, the low-resistant isolates accounted for 29.6%, and no high-resistant isolates were found.