响应面优化缅甸刺梧桐胶多糖制备工艺

Optimization of polysaccharide from myanmar’s Karaya gum using response surface methodology

以缅甸刺梧桐胶为原料,运用单因素试验结合响应面法对超声提取刺梧桐胶多糖的工艺进行优化,确定最佳工艺条件为:0.06mol/L的NaOH、超声时间1.52h、液料比100.16∶1(mL∶g),此条件下刺梧桐胶多糖得率的实际值为62.25%,预测值为62.7529%,实际值与预测值基本相符,证实该预测模型的实用性和准确性。因此,试验采用响应面法优化缅甸刺梧桐胶多糖的工艺准确可靠,可为后续利用发展刺梧桐胶提供一定的理论基础。

On the basis of single factor,the response surface method was used to optimize the ultrasonic extraction of total polysaccharide from Karaya gum.The optimal condition of ultrasonic extraction was as follows:NaOH concentration 0.06 mol/L,ultrasonic time 1.52 h and ratio of liquid to solid 100.16∶1(mL∶g).Under the above conditions,the yield of total polysaccharide was 62.25%,which was very close to the predicted value of 62.75%.Therefore,the accuracy of the model was confirmed.The result provided a theoretical basis for future comprehensive utilization of Karaya gum.